NON-ENZYMATIC, SALT-MEDIATED SYNTHESIS OF POLYNUCLEIC ACIDS
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Abstract
Provided herein is a method for synthesizing polynucleic acids, comprising the steps of (a) providing an acidic solution substantially free of nucleic acid polymerase and lipids, but containing mononucleotides and a monovalent salt; (b) drying and resolubilizing the mixture of step (a) a plurality of times; and (c) recovering polynucleic acids from a resolubilized mixture of step (b). In certain aspects, the method further uses a low pH, e.g. about 3; it can utilize monophosphates, such as AMP rather than ATP; and it can be used with a polynucleotide template to form a sequence at least partially complementary to said template. Thus, both single-stranded and double-stranded polynucleic acids are provided. Ammonia salts have been used to obtain RNA lengths from 10 to 300 nucleotides after 16 half hour cycles and an effective temperature includes between 80° C. and 100° C.
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Citations
40 Claims
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1-20. -20. (canceled)
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21. A system for preparing a polynucleotide from mononucleotides comprising:
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an acidic solution or a dried solid prepared from the acidic solution, free of polymerase and lipids, and comprising; a monovalent salt, wherein the monovalent salt is a metal halide salt or an ammonium halide salt at a concentration of at least 0.01 M; and mononucleotides selected from the group consisting of adenosine 5′
-monophosphate, uridine 5′
-monophosphate, guanosine 5′
-monophosphate, and cytidine-5′
-monophosphate, each at a concentration of from 0.001M to 3M,wherein the acidic solution or the dried solid is maintained in an anerobic environment. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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Specification