Vessel and method for cell isolation, culture and proliferation

• US 20190309245A1
• Filed: 12/24/2016
• Published: 10/10/2019
• Est. Priority Date: 12/26/2015
• Status: Active Application

• Current Assignee: Noboru Minowa
• Original Assignee: National Center For Geriatrics And Gerontology, Nepa Gene Co., Ltd.
• Inventors: Shimagaki, Masaaki, Suzuki, Kosho, Atsumi, Yusuke, Nakashima, Misako
• Application Number:
  US16/064,989
• Publication Number:
  US 20190309245A1
• Time in Patent Office: Days
• CPC Class Codes: C12M 23/04 Flat or tray type, drawers C12M23/10, C12M23/12, C12M23/16 take precedence
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/22 Transparent or translucent parts glassware for laboratory use B01L3/00
• CPC Class Codes: C12M 23/34 Internal compartments or partitions
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 25/04 in combination with well or multiwell plates, i.e. culture inserts
• CPC Class Codes: C12M 25/16 Particles; Beads; Granular material; Encapsulation chemical or physical processes conducted in the presence of fluids and solid particles B01J8/00
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696

Abstract

A sealable vessel for cell isolation, culture and proliferation comprising a cell separation unit, comprising a cell separation membrane having fine pores capable of isolating cells; a primary culture unit, having a culture surface on which cells having passed through the cell separation membrane can be cultured and a releasing structure through which cells and a liquid held inside the primary culture unit can be freely released toward a unit having a culture surface for a subsequent step; and a main vessel unit, having a culture surface for cell proliferation on which cells that have been released from a culture unit for a preceding step can be cultured, the main vessel unit having a structure in which the cell separation unit and all of the culture unit/units for preceding step/steps can be stored.

Claims

1. A sealable vessel for cell isolation, culture and proliferation, the vessel being a container-like structural body in which isolation, culture and proliferation of target cells of interest can be performed,

wherein (A) the vessel comprises a cell separation unit, a primary culture unit and a main vessel unit;

(B) the cell separation unit is a structural body comprising a cell separation membrane having fine pores capable of isolating cells;

(C) the primary culture unit is a container-like structural body that has a culture surface for primary culture on which cells that have passed through the cell separation membrane can be cultured and a releasing structure through which cells and a liquid that have been held inside the primary culture unit can be freely released toward a unit having a culture surface for a subsequent step; and

(D) the main vessel unit is a container-like structural body that has a culture surface for cell proliferation on which cells that have been released from a culture unit for a preceding step can be cultured, an area of the culture surface for cell proliferation being larger than that of a culture surface of the culture unit for the preceding step, the container-like structural body of the main vessel unit having a structure in which the cell separation unit and all of the culture unit/units for preceding step/steps can be stored.

2. The vessel for cell isolation, culture and proliferation according to claim 1,

wherein (b-1) the cell separation unit recited in (B) is a container-like structural body;

(b-2) the cell separation unit comprises the cell separation membrane having fine pores capable of isolating cells, the cell separation membrane being provided on at least a part of surfaces of the container-like structural body of the cell separation unit;

(c-1) the primary culture unit recited in (C) is a container-like structural body;

(c-2) the primary culture unit has a structure with which the cell separation unit can be disposed in a state in which, when a liquid is filled in the container-like structural body of the primary culture unit, a surface of the cell separation membrane and a surface of the liquid filled are in contact with each other;

(c-3) the primary culture unit has the culture surface for primary culture on which the cells that have passed through the cell separation membrane can be cultured, the culture surface for primary culture being provided on at least a part of surfaces of the container-like structural body of the primary culture unit;

(c-4) the primary culture unit has the releasing structure through which the cells and the liquid that have been held inside the container-like structural body of the primary culture unit can be freely released toward the unit having the culture surface for the subsequent step, the releasing structure being provided at a surface of the container-like structural body of the primary culture unit;

(d-1) the main vessel unit recited in (D) is a container-like structural body mainly composed of a main vessel and a main lid portion;

(d-2) the main vessel unit has the culture surface for cell proliferation on which the cells that have been released from the culture unit for the preceding step can be cultured, the area of the culture surface for cell proliferation being larger than that of the culture surface of the culture unit for the preceding step;

(d-3) the main vessel unit has a structure with which the cell separation unit and all of the culture unit/units for the preceding step/steps can be stored in the container-like structural body of the main vessel unit; and

(d-4) the main lid portion is a lid-like structural body with which the entire vessel can be sealed.

3. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the cell separation unit is a unit for isolating the target cells of interest into the container-like structural body of the primary culture unit through the cell separation membrane.

4. The vessel for cell isolation, culture and proliferation according to claim 1, wherein, when an area of the culture surface of the culture unit for the preceding step is taken as 1, a ratio of an area of the culture surface for cell proliferation is within a range of more than 1 to not more than 20.

5. The vessel for cell isolation, culture and proliferation according to claim 1, wherein, in the vessel for cell isolation, culture and proliferation,

(E)(e-1) at least a part of all the culture surfaces included in the vessel for cell isolation, culture and proliferation and at least a part of vessel structures opposed to or substantially opposed to the culture surface for cell proliferation of the main vessel unit are made of light-transmitting material;

and

(e-2) the culture surfaces inside the vessel can be observed through portions having light-transmitting properties without opening the main vessel unit.

6. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the primary culture unit is a container-like structural body composed of two or more members, and has a structure with which the releasing structure can be adjusted to open as the members of the container-like structural body of the primary culture unit are detached or their positions are changed.

7. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the primary culture unit has a structure with which the releasing structure can be adjusted to open in response to operation from outside the main vessel unit.

8. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the primary culture unit has a structure with which the releasing structure can be adjusted to open in response to operation of the main lid portion.

9. The vessel for cell isolation, culture and proliferation according to claim 1, wherein, in the vessel for cell isolation, culture and proliferation,

(F)(f-1) a structure through which a hollow tubular member can be inserted into or taken out of the main vessel without opening the main vessel unit is provided at a wall surface of the main vessel or the main lid portion of the main vessel unit;

(f-2) the structure is disposed at a position at which the liquid filled in the primary culture unit can be suctioned out through the hollow tubular member inserted; and

(f-3) the structure is mainly composed of an elastic member which can secure airtightness both in a state in which the hollow tubular member is not inserted and in a state in which the hollow tubular member is inserted.

10. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the cell separation unit has a structure with which a position of the cell separation membrane can be adjusted in response to operation of the main lid portion.

11. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the main vessel unit is provided with a gas exchange port.

12. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the main vessel unit is provided with a medium exchange port through which a medium can be introduced into or discharged from the main vessel.

13. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the cell separation unit is a container-like structural body that is mainly composed of the cell separation membrane and a membrane holding structure, and a peripheral portion of a bottom surface of an opening portion of the membrane holding structure covered by the cell separation membrane has a shape having an upward inclination toward the opening portion.

14. The vessel for cell isolation, culture and proliferation according to claim 1, comprising:

(G) one or more cell proliferation units with which a plurality of cell proliferation processes can be performed after a primary culture process;

wherein (g-1) the cell proliferation unit is a container-like structural body;

(g-2) the cell proliferation unit comprises a culture surface for cell proliferation on which cells that have been released from a culture unit for a preceding step can be cultured, an area of the culture surface for cell proliferation being larger than that of a culture surface of the culture unit for the preceding step;

(g-3) the cell proliferation unit has a structure with which the cell separation unit and all of the culture unit/units for the preceding step/steps can be stacked or stored in the container-like structural body of the cell proliferation unit; and

(g-4) the cell proliferation unit has a releasing structure through which cells and a liquid that have been held inside the container-like structural body of the cell proliferation unit can be freely released toward a unit having a culture surface for a subsequent step, the releasing structure being provided at a surface of the container-like structural body of the cell proliferation unit.

15. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the fine pores formed in the cell separation membrane have an average size of 1 to 20 μ

m, and the cell separation membrane has a porosity of 5 to 50%.

16. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the fine pores formed in the cell separation membrane are open in a vertical direction and/or in a substantially vertical direction with respect to a membrane surface.

17. The vessel for cell isolation, culture and proliferation according to claim 1, wherein a part or all of the fine pores formed in the cell separation membrane are arranged in a lattice pattern, a substantially lattice pattern, a staggered pattern, a substantially staggered pattern, a spiral pattern, a substantially spiral pattern, a concentric pattern, a substantially concentric pattern, or a combination of the aforementioned patterns.

18. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the cell separation membrane has a structure in which hydrophilic polymers are covalently bonded to a surface of a base material layer made of hydrophobic polymers.

19. The vessel for cell isolation, culture and proliferation according to claim 1,

wherein the cell separation membrane has a structure in which one or more selected from the hydroxyl group, the carboxyl group and a compound structure including said functional groups are covalently bonded to a vicinity of the surface of the base material layer made of hydrophobic polymers;

and/or

the cell separation membrane has an insoluble layer in the vicinity of the surface of the base material layer made of hydrophobic polymers, the insoluble layer being insoluble in a good solvent for the hydrophobic polymers.

20. The vessel for cell isolation, culture and proliferation according to claim 1, wherein a surface of the culture surface for primary culture and/or a surface of the culture surface for cell proliferation are modified with molecules that exhibit cell adhesion properties during culture and exhibits cell detachment properties in response to a stimulus.

21. The vessel for cell isolation, culture and proliferation according to claim 20, wherein the molecules that exhibit cell detachment properties are temperature responsive polymer molecules having a phase transition temperature, or a lower critical solution temperature, of 15 to 35°

C.

22. The vessel for cell isolation, culture and proliferation according to claim 1,

wherein the culture surface for primary culture and/or the culture surface for cell proliferation have a structure in which one or more selected from the hydroxyl group, the carboxyl group, and a compound structure including said functional groups are covalently bonded to a vicinity of a surface of a base material layer made of hydrophobic polymers;

and/or

the culture surface for primary culture and/or the culture surface for cell proliferation have an insoluble layer in the vicinity of the surface of the base material layer made of hydrophobic polymers, the insoluble layer being insoluble in a good solvent for the hydrophobic polymers.

23. The vessel for cell isolation, culture and proliferation according to claim 1,

wherein the structural bodies constituting the vessel for cell isolation, culture and proliferation have a structure in which one or more selected from the hydroxyl group, the carboxyl group, and a compound structure including said functional groups are covalently bonded to a vicinity of a surface of a base material layer made of hydrophobic polymers;

and/or

the structural bodies constituting the vessel for cell isolation, culture and proliferation have an insoluble layer provided in the vicinity of the surface of the base material layer made of hydrophobic polymers, the insoluble layer being insoluble in a good solvent for the hydrophobic polymers.

24. The vessel for cell isolation, culture and proliferation according to claim 1, wherein the vessel for cell isolation, culture and proliferation is a vessel for actively isolating stem cells and performing their proliferation.

25. A method for cell isolation, culture and proliferation, using the vessel for cell isolation, culture and proliferation according to claim 1.

26. The method for cell isolation, culture and proliferation according to claim 25, wherein the target cells of interest include stem cells.