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MULTIMODAL ASSAY FOR DETECTING NUCLEIC ACID ABERRATIONS

  • US 20190309352A1
  • Filed: 11/16/2017
  • Published: 10/10/2019
  • Est. Priority Date: 11/16/2016
  • Status: Abandoned Application
First Claim
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1. A method for determining a nucleotide sequence for one or more target nucleic acids of interest in a subject comprising:

  • a) obtaining a nucleic acid sample isolated from a subject;

    b) adding an anchor sequence to one of the 3′

    or 5′

    end of a plurality of nucleic acids from the sample in step a) to create an anchor product;

    c) hybridizing an anchor primer to the anchor product of step b), wherein the anchor primer is substantially complementary to the anchor sequence from step b), and hybridizing a genome-informed primer, which is substantially complementary to a repeat sequence in the nucleic acid, to produce a plurality of replicons, wherein the anchor sequence and the repeat sequence flank a gap region in the plurality of target nucleic acid sequences of interest;

    d) sequencing a plurality of amplicons that are amplified from the replicons in step c) to determine the nucleotide sequence of one or more target nucleic acids.

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