MICROFLUIDIC ORGANIC ELECTROCHEMICAL TRANSISTOR SENSORS FOR REAL TIME NITRIC OXIDE DETECTION

• US 20190310225A1
• Filed: 04/05/2019
• Published: 10/10/2019
• Est. Priority Date: 04/06/2018
• Status: Abandoned Application

• Current Assignee: Charles Stark Draper Laboratory Incorporated
• Original Assignee: Charles Stark Draper Laboratory Incorporated
• Inventors: Zhang, Hongmei
• Application Number:
  US16/376,264
• Publication Number:
  US 20190310225A1
• Time in Patent Office: Days
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0663 Whole sensors
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 33/0037 for NOx
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: H10K 10/462 Insulated gate field-effect transistors [IGFETs]
• CPC Class Codes: H10K 85/1135 Polyethylene dioxythiophene [PEDOT]; Derivatives thereof

Abstract

The present disclosure describes a solution to rapidly detect trace amounts of biomarkers present in a fluid sample. The solution can be, for example, used to diagnosis sepsis through the detection of nitric oxide. The solution includes one or more organic electrochemical transistors that are functionalized with a bio-recognition coating. The bio-recognition coating can bind or otherwise interact with the biomarkers to change the transconductance of the organic electrochemical transistors. The solution can detect the change in the transconductance of the organic electrochemical transistors and signal the presence of the biomarker.

Claims

1. A microfluidic device, comprising:

a fluid chamber to hold a sample fluid; and

a biomarker sensor at least partially disposed in a wall defining the fluid chamber;

the biomarker sensor comprising;

a drain electrode;

a source electrode;

a channel material electrically coupling the drain electrode and the source electrode; and

a functional coating disposed on a surface of the channel material, the functional coating configured to bind with a biomarker in the sample fluid and, responsive to the binding of the biomarker with the functional coating, change a conductivity of the channel material.

2. The device of claim 1, wherein the channel material comprises poly(3,4-ethylenedioxythiophene) polystyrene sulfonate.

3. The device of claim 1, wherein the functional coating comprises hemin.

4. The device of claim 3, wherein the biomarker is nitric oxide.

5. The device of claim 1, wherein the functional coating comprises an aptamer or an antibody.

6. The device of claim 1, wherein the channel material between the drain electrode and the source electrode has a length between 5 μ

m and 50 μ

m and a width between 25 μ

m and 125 μ

m.

7. The device of claim 1, further comprising a microfluidic flow channel fluidically coupled with the fluid chamber, the microfluidic flow channel comprising at least one separation region to remove undesirable particles from the fluid sample.

8. A microfluidic device, comprising:

a fluid chamber to hold a sample fluid; and

a biomarker sensor at least partially disposed in a wall defining the fluid chamber;

the biomarker sensor comprising;

a drain electrode;

a source electrode;

a channel material electrically coupling the drain electrode and the source electrode;

a gate electrode; and

a functional coating disposed on a surface of the gate electrode, the functional coating configured to bind with a biomarker in the sample fluid and, responsive to the binding of the biomarker with the functional coating, change a gate voltage of the gate electrode.

9. The device of claim 8, wherein the channel material comprises poly(3,4-ethylenedioxythiophene) polystyrene sulfonate.

10. The device of claim 8, wherein the functional coating comprises hemin.

11. The device of claim 10, wherein the biomarker is nitric oxide.

12. The device of claim 8, wherein the functional coating comprises an aptamer or an antibody.

13. The device of claim 8, further comprising a microfluidic flow channel fluidically coupled with the fluid chamber, the microfluidic flow channel comprising at least one separation region to remove undesirable particles from the fluid sample

14. A method, comprising:

providing a microfluidic device comprising;

a fluid chamber; and

a biomarker sensor at least partially disposed in a wall defining the fluid chamber;

the biomarker sensor comprising;

a drain electrode;

a source electrode;

a channel material electrically coupling the drain electrode and the source electrode; and

a functional coating disposed on a surface of the channel material, the functional coating configured to bind with a biomarker in the sample fluid and, responsive to the binding of the biomarker with the functional coating, change a conductivity of the channel material;

flowing a fluid through the microfluidic device and at least partially over the biomarker sensor;

measuring a conductivity through the channel material; and

determining an amount of the biomarker in the fluid based on the measured conductivity through the channel material.

15. The method of claim 14, wherein the channel material comprises poly(3,4-ethylenedioxythiophene) polystyrene sulfonate.

16. The method of claim 14,wherein the functional coating comprises hemin.

17. The method of claim 16, wherein the biomarker is nitric oxide.

18. The method of claim 14,wherein the functional coating comprises an aptamer or an antibody.

19. The method of claim 14, further comprising applying at least one acoustic wave to the fluid flowing through the microfluidic device to drive a plurality of undesirable particles in the fluid toward an outlet of the microfluidic device.

20. The method of claim 19, wherein the undesirable particles comprise toxins, bacteria, viruses, erythrocytes, leukocytes, or thrombocytes.