SYSTEMS AND METHODS FOR MEASURING WATER AND LIPID CONTENT IN TISSUE SAMPLES
First Claim
1. A method for measuring water and lipid content in a tissue sample, the method comprising:
- generating, using a light source and a spatial modulation device, a first plurality of patterns at a first spatial frequency on a tissue sample plane and a second plurality of patterns at a second spatial frequency on the tissue sample plane for a first wavelength of light from a plurality of sequential wavelengths of light, the light source being configured to emit the plurality of sequential wavelengths of light within a predetermined range of wavelengths;
obtaining, using an imaging device, (i) first image data reproducible as images of the first plurality of patterns at the first spatial frequency for the first wavelength of light and (ii) second image data reproducible as images of the second plurality of patterns at the second spatial frequency for the first wavelength of light;
generating, using a controller, a first demodulated image for the first wavelength of light based on the first image data;
determining, using the controller, a first diffuse reflectance value for each of a plurality of locations on the tissue sample plane at the first wavelength of light based on the first demodulated image;
generating, using the controller, a second demodulated image for the first wavelength of light based on the second image data;
determining, using the controller, a second diffuse reflectance value for each of the plurality of locations on the tissue sample plane for the first wavelength of light based on the second demodulated image; and
determining, using the controller, based on the first diffuse reflectance value and the second diffuse reflectance value, (i) a first optical property and (ii) a second optical property for each of the plurality of locations on the tissue sample plane for the first wavelength of light.
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Abstract
An imaging system for measuring water and blood lipid content in a tissue sample includes a light source configured to emit a plurality of sequential wavelengths of light within a predetermined range of wavelengths, a spatial modulation device configured to direct each of the plurality of sequential wavelengths of light onto a tissue sample plane to generate a first plurality of patterns on the issue sample plane at a first spatial frequency and a second plurality of patterns on the tissue sample plane at a second spatial frequency, an imaging device configured to generate first image data reproducible as images the first plurality of patterns and second image data reproducible as images the second plurality of patterns, and a controller configured to determine a first optical property and a second optical property for each location on the sample plane.
6 Citations
28 Claims
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1. A method for measuring water and lipid content in a tissue sample, the method comprising:
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generating, using a light source and a spatial modulation device, a first plurality of patterns at a first spatial frequency on a tissue sample plane and a second plurality of patterns at a second spatial frequency on the tissue sample plane for a first wavelength of light from a plurality of sequential wavelengths of light, the light source being configured to emit the plurality of sequential wavelengths of light within a predetermined range of wavelengths; obtaining, using an imaging device, (i) first image data reproducible as images of the first plurality of patterns at the first spatial frequency for the first wavelength of light and (ii) second image data reproducible as images of the second plurality of patterns at the second spatial frequency for the first wavelength of light; generating, using a controller, a first demodulated image for the first wavelength of light based on the first image data; determining, using the controller, a first diffuse reflectance value for each of a plurality of locations on the tissue sample plane at the first wavelength of light based on the first demodulated image; generating, using the controller, a second demodulated image for the first wavelength of light based on the second image data; determining, using the controller, a second diffuse reflectance value for each of the plurality of locations on the tissue sample plane for the first wavelength of light based on the second demodulated image; and determining, using the controller, based on the first diffuse reflectance value and the second diffuse reflectance value, (i) a first optical property and (ii) a second optical property for each of the plurality of locations on the tissue sample plane for the first wavelength of light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. An imaging system for measuring water and blood lipid content in a tissue sample, the imaging system comprising:
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a light source configured to emit a plurality of sequential wavelengths of light at a predetermined interval, each of the plurality of sequential wavelengths of light being within a predetermined range of wavelengths; a spatial modulation device configured to direct each of the plurality of sequential wavelengths of light emitted from the light source onto a tissue sample plane and cause each of the plurality of sequential wavelengths of light to generate;
(i) a first plurality of patterns on the issue sample plane at a first spatial frequency, and (ii) a second plurality of patterns on the tissue sample plane at a second spatial frequency;an imaging device configured to generate (i) first image data reproducible as images of each of the first plurality of patterns and (ii) second image data reproducible as images each of the second plurality of patterns; and a controller including one or more processors and one or more memory devices, at least one of the one or more memory devices storing computer-readable instructions configured to cause at least one of the one or more processors to; generate, using the first image data, a first demodulated image associated with the first spatial frequency for each of the plurality of sequential wavelengths of light; determine a first diffuse reflectance value for each of a plurality of locations on the tissue sample plane for each of the plurality of sequential wavelength based on the first demodulated image; generate, using the second image data, a second demodulated image associated with the second spatial frequency for each of the plurality of sequential wavelengths of light; determine a second diffuse reflectance value for each of the plurality of locations on the tissue sample plane for each of the plurality of sequential wavelength based on the second demodulated image; and determine, based on the first diffuse reflectance value and the second diffuse reflectance value, (i) a first optical property and (ii) a second optical property for each of the plurality of locations on the sample plane for each of the plurality of sequential wavelengths of light. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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Specification