Isolated Bacterial Strain of the Genus Burkholderia and Pesticidal Metabolites Therefrom

• US 20190313645A1
• Filed: 06/24/2019
• Published: 10/17/2019
• Est. Priority Date: 02/25/2010
• Status: Active Grant

• Current Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Original Assignee: Marrone Bio Innovations Inc. (Bioceres Crop Solutions Corp.)
• Inventors: Asolkar, Ratnakar, Koivunen, Marja, Marrone, Pamela G., Cordova-Kreylos, Ana Lucia, Huang, Huazhang
• Granted Patent: US 11,382,331 B2
• Time in Patent Office: Days
• CPC Class Codes: A01N 43/00 Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds containing cyclic anhydrides, cyclic imides A01N37/00; containing compounds of the formula containing only one heterocyclic ring, wherein m>=1 and n>=0 and is unsubstituted or alkylsubstituted pyrrolidine, piperidine, morpholine, thiomorpholine, piperazine or a polymethyleneimine with four or more CH2 groups, A01N33/00 - A01N41/12; containing cyclopropane carboxylic acids or derivatives thereof, e.g. esters having heterocyclic rings, A01N53/00
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A01N 43/74 five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/76 1,3-Oxazoles; Hydrogenated 1,3-oxazoles
• CPC Class Codes: A01N 43/86 six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,3
• CPC Class Codes: A01N 43/90 having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
• CPC Class Codes: A01N 57/20 containing acyclic or cycloaliphatic radicals
• CPC Class Codes: A01N 63/20 Bacteria; Substances produced thereby or obtained therefrom
• CPC Class Codes: C07D 263/32 with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 263/34 with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
• CPC Class Codes: C07D 309/14 Nitrogen atoms not forming part of a nitro radical
• CPC Class Codes: C07D 407/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 413/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 413/06 linked by a carbon chain containing only aliphatic carbon atoms
• CPC Class Codes: C07D 493/10 Spiro-condensed systems
• CPC Class Codes: C07D 498/14 Ortho-condensed systems
• CPC Class Codes: C07D 513/04 Ortho-condensed systems
• CPC Class Codes: C12N 1/00 Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 17/04 : containing a five-membered hetero ring, e.g. griseofulvin , vitamin C
• CPC Class Codes: C12P 17/06 : containing a six-membered hetero ring, e.g. fluorescein
• CPC Class Codes: C12P 17/14 : Nitrogen or oxygen as hetero atom and at least one other diverse hetero ring atom in the same ring
• CPC Class Codes: C12P 17/16 : containing two or more hetero rings thiamine open chain analogs C12P17/167, i.e. not condensed among themselves or through a common carbocyclic ring system
• CPC Class Codes: C12P 17/18 : containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin e.g. Rifamycin C12P17/189
• CPC Class Codes: C12R 2001/00 : Microorganisms ; Processes using microorganisms
• CPC Class Codes: C12R 2001/01 : Bacteria or Actinomycetales ; using bacteria or Actinomycetales

Abstract

A species of Burkholderia sp with no known pathogenicity to vertebrates but with pesticidal activity (e.g., plants, insects, fungi, weeds and nematodes) is provided. Also provided are natural products derived from a culture of said species and methods of controlling pests using said natural products.

Claims

1-21. -21. (canceled)

22. A method for inhibiting fungi growth in a plant comprising:

applying to said plant an effective amount of a fermented composition comprising whole cell broth collected from Burkholderia A396 (NRRL Accession No. B-50319) fermentation, wherein said fermented composition comprises FR901228 to inhibit said fungi growth.

23. The method of claim 22, wherein said composition further comprises a carrier, diluent, surfactant, and/or adjuvant.

25. The method of claim 22, wherein said fungi comprises Botrytis cinereal or Phytophtora sp.

26. The method of claim 22, further comprising applying a second fungicidal agent sub stance.

27. The method of claim 26, wherein said second fungicidal agent comprises paraffinic oil, tea tree oil, lemongrass oil, clove oil, cinnamon oil, citrus oil, and/or rosemary oil.

28. The method of claim 26, wherein said second fungicidal agent comprises benzimidazole, demethylation inhibitor, morpholine, hydroxyprimidine, anilinopyrimidine, phosphorothilolate, quinone outside inhibitor, quinoline, dicarboximide, carboxamide, phenylamide, anilinopyrimidine, phenylpyrrole, aromatic hydrocarbon, cinnamic acid, hydroxyanilide, antibiotic, polyoxin, acylamine, phthalimide, and/or benzenoid (xy-lylalanine).

29. The method of claim 26, wherein said second fungicidal agent substance is applied in rotation.