Magnetic actuated microscaffold for minimally invasive osteochondral regeneration

• US 20190314156A1
• Filed: 05/09/2018
• Published: 10/17/2019
• Est. Priority Date: 04/16/2018
• Status: Active Grant

• Current Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Original Assignee: Industry Foundation Of Chonnam National University (Chonnam National University)
• Inventors: Park, Jongoh, Choi, Eunpyo, Kim, Chang-Sei, Go, Gwangjun, Yoo, Ami, Zhen, Jin
• Granted Patent: US 11,452,605 B2
• Time in Patent Office: Days
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61F 2002/30062 (bio)absorbable, biodegradable, bioerodable, (bio)resorbable, resorptive
• CPC Class Codes: A61F 2002/30766 Scaffolds for cartilage ingrowth and regeneration
• CPC Class Codes: A61K 31/728 Hyaluronic acid
• CPC Class Codes: A61L 2300/406 Antibiotics
• CPC Class Codes: A61L 2300/414 Growth factors
• CPC Class Codes: A61L 2400/12 Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
• CPC Class Codes: A61L 2400/18 Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 2430/06 for cartilage reconstruction, e.g. meniscus
• CPC Class Codes: A61L 2430/24 for joint reconstruction
• CPC Class Codes: A61L 27/16 obtained by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/222 Gelatin
• CPC Class Codes: A61L 27/225 Fibrin; Fibrinogen
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3654 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/3817 Cartilage-forming cells, e.g. pre-chondrocytes
• CPC Class Codes: A61L 27/3821 Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3852 Cartilage, e.g. meniscus
• CPC Class Codes: A61L 27/44 : having a macromolecular matrix
• CPC Class Codes: A61L 27/50 : Materials characterised by their function or physical properties , e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
• CPC Class Codes: A61L 27/54 : Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 : Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 27/58 : Materials at least partially resorbable by the body
• CPC Class Codes: C08L 29/04 : Polyvinyl alcohol; Partially hydrolysed homopolymers or copolymers of esters of unsaturated alcohols with saturated carboxylic acids
• CPC Class Codes: C08L 67/04 : Polyesters derived from hydroxycarboxylic acids, e.g. lactones C08L67/06 takes precedence
• CPC Class Codes: C08L 71/02 : Polyalkylene oxides

Abstract

Provided is a magnetically actuated microscaffold for minimal invasive osteochondral regeneration. More particularly, provided is a composition for cartilage regeneration, a microscaffold for cartilage regeneration, in which magnetic particles and cartilage regeneration cells are loaded on the surface of or within a 3-dimensional porous microstructure composed of a biodegradable polymer and having a diameter of 200-300 μm; and a microscaffold for bone regeneration, in which magnetic particles and bone regeneration cells are loaded on the surface of or within a 3-dimensional porous microstructure composed of a biodegradable polymer and having a diameter of 700-900 μm.

Claims

1. A composition for cartilage regeneration, comprising:

a microscaffold for cartilage regeneration, in which magnetic particles and cartilage regeneration cells are loaded on the surface of or within a 3-dimensional porous microstructure composed of a biodegradable polymer and having a diameter of 200-300 μ

m; and

a microscaffold for bone regeneration, in which magnetic particles and bone regeneration cells are loaded on the surface of or within a 3-dimensional porous microstructure composed of a biodegradable polymer and having a diameter of 700-900 μ

m.

2. The composition of claim 1, wherein the biodegradable polymer is polyvinyl alcohol, polyethylene glycol, poly(lactic-co-glycolic acid) (PLGA), polylactic acid (PLA), polyglycolic acid (PGA), polyhydroxyalkanoate (PHA), polycaprolactone (PCL), or collagen.

3. The composition of claim 1, wherein the microscaffold for cartilage regeneration has an inner pore size of 45-115 μ

m.

4. The composition of claim 1, wherein the microscaffold for bone regeneration has an inner pore size of 85-130 μ

m.

5. The composition of claim 1, wherein the magnetic particle is magnetite or maghemite.

6. The composition of claim 1, wherein the cartilage regeneration cells are stem cells or chondrocytes.

7. The composition of claim 1, wherein the bone regeneration cells are stem cells or osteoblasts.

8. The composition of claim 1, wherein the composition further comprises a drug for cartilage regeneration.

9. The composition of claim 8, wherein the drug for cartilage regeneration is a cell differentiation growth factor or an antibiotic.

10. The composition of claim 9, wherein the cell differentiation growth factor is TGF-β

or BMPs.

11. A method of cartilage regeneration for an individual, comprising:

administering the composition for cartilage regeneration of claim 1 to the individual in need of cartilage regeneration.

12. The method of claim 11, further comprising transporting the composition for cartilage regeneration to an affected area using an external magnetic field.

13. The method of claim 11, wherein the composition for cartilage regeneration is administered by a joint cavity injection.

14. The method of claim 11, wherein the microscaffold for cartilage regeneration and the microscaffold for bone regeneration are administered at a ratio of 1:

3 to 3;

1.