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COMPOSITIONS AND METHODS COMPRISING IMPROVEMENTS OF CRISPR GUIDE RNAS USING THE H1 PROMOTER

  • US 20190314521A1
  • Filed: 07/05/2017
  • Published: 10/17/2019
  • Est. Priority Date: 07/05/2016
  • Status: Active Grant
First Claim
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1. A non-naturally occurring nuclease system comprising one or more vectors comprising:

  • a) a promoter operably linked to at least one nucleotide sequence encoding a nuclease system guide RNA (gRNA), wherein the gRNA hybridizes with a target sequence of a DNA molecule or RNA molecule in a cell, and wherein the DNA molecule or RNA encodes one or more gene products expressed in the cell; and

    b) a regulatory element operable in a cell operably linked to a nucleotide sequence encoding a genome-targeted nuclease,wherein components (a) and (b) are located on the same or different vectors of the system, wherein the gRNA targets and hybridizes with the target sequence and the nuclease cleaves the DNA molecule or RNA to alter expression of the one or more gene products.

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