MIXING DEVICE FOR CELL PRINTING COMPOSITION

• US 20190314778A1
• Filed: 06/24/2019
• Published: 10/17/2019
• Est. Priority Date: 12/23/2016
• Status: Active Grant

• Current Assignee: Korea Polytechnic University Industry Academic Cooperation Foundation, T&R Biofab Co., Ltd.
• Original Assignee: Korea Polytechnic University Industry Academic Cooperation Foundation, T&R Biofab Co., Ltd.
• Inventors: Ahn, Geunseon, Shim, Jinhyung, Lee, Jeongseok, Lee, lngyu, Min, Kyunghyun, Jin, Songwan, Yun, Wonsoo
• Granted Patent: US 10,874,999 B2
• Time in Patent Office: Days
• CPC Class Codes: B01F 2101/44 Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
• CPC Class Codes: B01F 27/90 with paddles or arms
• CPC Class Codes: B01F 27/92 with helices or screws
• CPC Class Codes: B01F 35/32005 Type of drive
• CPC Class Codes: B01F 35/33 Transmissions; Means for modifying the speed or direction of rotation
• CPC Class Codes: B01F 35/50 Mixing receptacles
• CPC Class Codes: B01F 35/7174 using pistons, plungers or syringes
• CPC Class Codes: B05C 17/00566 with a dynamic mixer in the nozzle
• CPC Class Codes: B05C 17/0133 Nut and bolt advancing mechanism, e.g. threaded piston rods
• CPC Class Codes: B33Y 30/00 Apparatus for additive manufacturing; Details thereof or accessories therefor
• CPC Class Codes: B33Y 40/10 Pre-treatment
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12M 33/00 Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00

Abstract

The mixing device of cell printing composition according to the present disclosure comprises a first syringe comprising a first container for containing first liquid, a first moving element discharging the first liquid contained in the first container; a second syringe comprising a second container for containing second liquid, a second moving element discharging the second liquid contained in the second container; a mixing container which comprises a rotating mixing unit and receives the discharged first liquid and the discharged second liquids; a power transmission unit for driving the first moving element, the second moving element and the mixing unit; a first threaded guide where the first moving element is movably connected; a second threaded guide where the second moving element is movably connected; and a rod for rotating the mixing unit.

Claims

1. A mixing device of cell printing composition comprising:

a first syringe comprising a first container for containing first liquid, a first moving element discharging the first liquid contained in the first container;

a second syringe comprising a second container for containing second liquid, a second moving element discharging the second liquid contained in the second container;

a mixing container comprising a rotating mixing unit, the mixing container receiving the discharged first liquid and the discharged second liquid;

a power transmission unit for driving the first moving element, the second moving element and the mixing unit;

a first threaded guide where the first moving element is movably connected;

a second threaded guide where the second moving element is movably connected; and

a rod for rotating the mixing unit;

wherein the first moving element moves by the rotation of the first threaded guide;

the second moving element moves by the rotation of the second threaded guide; and

the first threaded guide, the second threaded guide, and the rod are rotated by the power transmission unit.

2. The mixing device according to claim 1, wherein the power transmission unit comprises a ring gear, a sun gear which is rotatable by the rotation of the ring gear, a first planetary gear which is engageable with the sun gear, and a second planetary gear which is engageable with the sun gear;

and wherein the first threaded guide is rotatable by the first planetary gear;

the second threaded guide is rotatable by the second planetary gear; and

the rod is rotatable by the sun gear.

3. The mixing device according to claim 2, wherein the first moving element is connected to the first threaded guide via a first connecting portion which is guidable by the first threaded guide;

and the second moving element is connected to the second threaded guide via a second connecting portion which is guidable by the second threaded guide.

4. The mixing device according to claim 3, further comprising a first guard portion having a first slot where the first connecting portion is guidable;

and a second guard portion having a second slot where the second connecting portion is guidable.

5. The mixing device according to claim 1, wherein the mixing unit comprises a second mixing portion comprising a spiral portion extending up and down direction.

6. The mixing device according to claim 5, wherein the second mixing portion further comprises a shaft extending up and down direction;

the spiral portion extends in the longitudinal direction of the shaft; and

the shaft is connected to the rod and is rotatable by the rotation of the rod.

7. The mixing device according to claim 1, wherein the first moving element is slidable within the first container;

and the second moving element is slidable within the second container.

8. The mixing device according to claim 4, wherein the first connecting portion comprises a first groove where the first guard is fit;

and the second connecting portion comprises a second groove where the second guard is fit.

9. The mixing device according to claim 1, wherein any one of the first liquid and the second liquid is cell culture fluid and the other is hydrogel having a sufficient viscosity for cell printing.