A METHOD OF ENHANCING THE PERFORMANCE OF CHROMATOGRAPHY METHODS FOR PURIFICATION OF PROTEINS
First Claim
1. A method of removing contaminants from a sample comprising a desired protein, the method comprising:
- (a) contacting the sample with (i) an amount of allantoin in excess of saturation, (ii) a cationic polymer and (iii) a fatty acid comprising 6 to 10 carbon atoms, thereby forming a mixture; and
(b) removing solids from the mixture.
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Accused Products
Abstract
This application describes compositions, kits, and methods for removing contaminants from a protein preparation, for example a cell culture harvest comprising a recombinant protein. The methods described herein can enhance the performance of a later applied chromatographic purification step. The method generally involves contacting a protein preparation comprising a desired protein with a combination of allantoin, a cationic polymer, and a fatty acid, thereby causing the formation of solids and removal of the solids using a suitable process. Further treatment by contacting the treated sample with a chemically reactive surface further enhances results achieved by the first purification step.
1 Citation
22 Claims
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1. A method of removing contaminants from a sample comprising a desired protein, the method comprising:
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(a) contacting the sample with (i) an amount of allantoin in excess of saturation, (ii) a cationic polymer and (iii) a fatty acid comprising 6 to 10 carbon atoms, thereby forming a mixture; and (b) removing solids from the mixture. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22)
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21. An aqueous composition comprising (a) an amount of allantoin in excess of saturation, (b) a cationic polymer at a concentration of in a range of 0.001% w/v to 0.2% w/v, wherein the cationic polymer is selected from polyallylamine, chitosan, polydiallyldimethylammonium chloride (pDADMAC), DEAE-cellulose and DEAE-dextran, (c) a fatty acid at a concentration of 0.25% (vol/vol) to about 0.55% (vol/vol), wherein the fatty acid is selected from caprylic acid, heptanoic acid, heptenoic acid, octenoic acid, nonanoic acid, nonenoic acid, and decanoic acid, and (d) an antibody, a binding fragment thereof or a portion thereof.
Specification