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METHODS FOR ISOLATING AND CULTURING LIVING CELLS USING METHOD OF PERMEABILIZING CELL MEMBRANE

  • US 20190316092A1
  • Filed: 04/12/2019
  • Published: 10/17/2019
  • Est. Priority Date: 04/13/2018
  • Status: Abandoned Application
First Claim
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1. A method of isolating target cells in a living state, comprising:

  • (s1) treating a test material including target cells with a streptococcal hemolytic exotoxin, i.e., Streptolysin O (SLO);

    (s2) treating the test material with an antibody against a target intracellular protein of the target cells; and

    (s3) isolating the target cells in a living state, which have a positive response to the antibody.

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