COMPOSITIONS AND METHODS FOR MOLECULAR LABELING
2 Assignments
0 Petitions
Accused Products
Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
-
Citations
45 Claims
-
1-31. -31. (canceled)
-
32. A method for analyzing a nucleic acid, the method comprising:
-
obtaining nucleic acid from a cell or a population of cells; exposing the nucleic acid to a transposase to form a reaction product; introducing the reaction product to a droplet comprising barcoded primers and reagents for amplification; conducting an amplification reaction in the droplet to produce barcoded amplicons corresponding to transposase-accessible regions of the nucleic acid; and sequencing the transposase-accessible regions. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
-
Specification