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METHOD FOR THE INACTIVATION AND INACTIVATION TESTING OF XENO ANTIGENS IN FOODS OF ANIMAL ORIGIN, PARTICULARLY FOR MILK AND DERIVATIVES, AND IN FOODS OF PLANT ORIGIN, PARTICULARLY FOR MILK SUBSTITUTES BASED ON SOY AND/OR RICE

  • US 20190317069A1
  • Filed: 11/14/2017
  • Published: 10/17/2019
  • Est. Priority Date: 11/16/2016
  • Status: Active Grant
First Claim
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1. A method for the inactivation and inactivation testing of xenoantigens in foods of vegetable and animal origin, characterized in that it comprises the following steps:

  • making up a solution with a food of vegetable or animal origin as a solvent and one or more phenolic compounds, polyphenolic compounds and derivatives thereof, comprising phenylpropanoids, as a solute, for the inactivation of at least part of the xenogeneic epitopes from said food;

    incubating samples of said food of vegetable or animal origin with the addition of an antibody aimed at a xenoantigen epitope that is present in said food;

    separating the resulting immune complex created owing to the bond between antigen and antibody;

    preparing a well plate for the E.L.I.S.A. test with coating comprising said xenoantigen epitope,adding, in said wells, supernatant taken from said samples, said supernatant containing the part of anti-xenoantigen antibody that has not bonded with epitopes, a column of wells being adapted to define a reference value that corresponds to the maximum signal between antibody and epitopes,completing said plate with a secondary antibody conjugated with an enzyme, or other molecule, adapted to chromatically highlight any presence of anti-xenoantigen antibody,reading said plate, determining the presence of anti-xenoantigen antibody that has remained free in the solutions of the samples,comparing the absorbance values detected in the column that defines the reference values with the values detected in the other columns of samples of the plate.

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