DISEASE PATHWAY-BASED METHOD TO GENERATE BIOMARKER PANELS TAILORED TO SPECIFIC THERAPEUTICS FOR INDIVIDUALIZED TREATMENTS
First Claim
1. A method of identifying a cancer type of interest, comprising:
- (a) establishing a cellular model of cancer space based on two or more cell lines each having one or more disease signaling pathways causally involved in a cancer type of interest;
(b) treating the two or more cell lines with one or more exogenous agents;
(c) using phosphor-antibodies (P-Abs) to analyze phosphopathways of one or more biomarkers before and after treatment with the exogenous agents;
(d) determining the effect of the exogenous agents on an activity state of the one or more disease signaling pathways in the cell lines by identifying at least one of;
(i) abundance of one or more biomarkers, and (ii) post-translational modifications of one or more biomarkers, before and after treatment with the one or more exogenous agents through phosphopathway analysis using phosphor-antibodies (P-Abs);
(e) deriving one or more biomarkers for an activity state of the one or more disease signaling pathways in the cell lines from the one or more biomarkers; and
(f) defining the cancer type based on the one or more disease signaling pathways and responsiveness of the pathways to the one or more exogenous agents.
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Abstract
The increased efficacy and reduced unwanted side effects of drugs can be insured by treating only responsive patients. In an embodiment of the invention, signaling pathways that a particular drug interferes with, are derive together with predictive biomarkers and dynamic biomarker that can read the activity of these pathways before and after drug treatment in order to select a responder patient population. In an alternative embodiment of the invention, certain core pathways that the drug does not interfere with and that are known to be causally involved in a particular disease(s) can be identified, and derive the biomarkers for those to be able to exclude these patients that suffer from a disease in which those drug non effected pathways are involved from being treated with the specific drug in question.
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8 Claims
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1. A method of identifying a cancer type of interest, comprising:
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(a) establishing a cellular model of cancer space based on two or more cell lines each having one or more disease signaling pathways causally involved in a cancer type of interest; (b) treating the two or more cell lines with one or more exogenous agents; (c) using phosphor-antibodies (P-Abs) to analyze phosphopathways of one or more biomarkers before and after treatment with the exogenous agents; (d) determining the effect of the exogenous agents on an activity state of the one or more disease signaling pathways in the cell lines by identifying at least one of;
(i) abundance of one or more biomarkers, and (ii) post-translational modifications of one or more biomarkers, before and after treatment with the one or more exogenous agents through phosphopathway analysis using phosphor-antibodies (P-Abs);(e) deriving one or more biomarkers for an activity state of the one or more disease signaling pathways in the cell lines from the one or more biomarkers; and (f) defining the cancer type based on the one or more disease signaling pathways and responsiveness of the pathways to the one or more exogenous agents. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of classifying cancer, comprising:
identifying an alteration involved in a disease pathway. - View Dependent Claims (8)
Specification