BLOOD ANALYSIS METHOD AND BLOOD TEST KIT

• US 20190320961A1
• Filed: 06/26/2019
• Published: 10/24/2019
• Est. Priority Date: 12/28/2016
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Inventors: URANO, Hikaru, NAKATSUGAWA, Haruyasu, SUGIMOTO, Shinya, YONEKUBO, Isao
• Granted Patent: US 11,179,081 B2
• Time in Patent Office: Days
• CPC Class Codes: A61B 5/1405 Devices for taking blood samples devices for taking samples of other body liquids A61B10/0045
• CPC Class Codes: A61B 5/150343 Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes sampling from within body cavities A61B5/150015, A61B5/150045
• CPC Class Codes: A61B 5/150351 Caps, stoppers or lids for sealing or closing a blood collection vessel or container, e.g. a test-tube or syringe barrel caps for bottles or containers in general B65D41/00
• CPC Class Codes: A61B 5/150755 Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/042 Caps; Plugs
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: G01N 1/12 Dippers; Dredgers
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 33/96 involving blood or serum control standard

Abstract

An object of the present invention is to provide a blood analysis method and a blood test kit, which are for performing quantitative analysis of components by precisely obtaining a dilution factor. According to the present invention, provided is a blood analysis method including: a step of diluting a collected blood sample with a diluent solution; a step of determining a dilution factor by using a normal value of a normal component which is homeostatically present in blood; and a step of analyzing a concentration of a target component in the blood sample, in which the blood analysis method uses a member selected from the group consisting of a first storing instrument for storing the diluent solution, a collection instrument for collecting the blood, a separation instrument for separating and recovering blood plasma from the blood sample diluted with the diluent solution, a holding instrument for holding the separation instrument, and a second storing instrument for storing the recovered blood plasma, and in which the dilution factor is corrected after calculating in advance an amount of the normal component derived from the diluent solution, and/or an amount of the normal component derived from at least one of the members which may be contained in the diluent solution.

Claims

1. A blood analysis method, comprising:

a step of diluting a collected blood sample with a diluent solution;

a step of determining a dilution factor by using a normal value of a normal component which is homeostatically present in blood; and

a step of analyzing a concentration of a target component in the blood sample,

wherein the blood analysis method uses a member selected from the group consisting of

a first storing instrument for storing the diluent solution,

a collection instrument for collecting the blood,

a separation instrument for separating and recovering blood plasma from the blood sample diluted with the diluent solution,

a holding instrument for holding the separation instrument, and

a second storing instrument for storing the recovered blood plasma, and

wherein the dilution factor is corrected after calculating in advance an amount of the normal component derived from the diluent solution, and/or an amount of the normal component derived from at least one of the members which may be contained in the diluent solution.

2. The blood analysis method according to claim 1, wherein at least one of the members is the separation instrument.

3. The blood analysis method according to claim 2, wherein the separation instrument is composed of glass fiber.

4. The blood analysis method according to claim 1, wherein the normal component which is homeostatically present in blood is sodium ions or chloride ions.

5. The blood analysis method according to claim 1, wherein the normal component which is homeostatically present in blood is sodium ions or chloride ions, and another normal component which is homeostatically present in blood.

6. The blood analysis method according to claim 5, wherein the other normal component is total protein or albumins.

7. The blood analysis method according to claim 1, further comprising:

a step of transporting the diluent solution with which the blood sample is diluted, after the step of diluting the collected blood sample with the diluent solution,

wherein the dilution factor is corrected after calculating in advance an amount of the normal component derived from the diluent solution, and/or an amount of the normal component derived from at least one of the members which may be contained in the diluent solution, in consideration of an influence of an environmental history of the transportation step.

8. The blood analysis method according to claim 1, wherein a volume of the diluent solution is four or more times a volume of the blood plasma.

9. A blood test kit used in the blood analysis method according to claim 1, the blood test kit comprising:

a first storing instrument for storing a diluent solution;

a collection instrument for collecting blood;

a separation instrument for separating and recovering blood plasma from a blood sample diluted with the diluent solution;

a holding instrument for holding the separation instrument; and

a second storing instrument for storing the recovered blood plasma.