CATALASE INHIBITOR AND METHOD FOR MEASURING ANALYTE USING CATALASE INHIBITOR

• US 20190321335A1
• Filed: 06/20/2017
• Published: 10/24/2019
• Est. Priority Date: 06/21/2016
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: PHC Holdings Corporation
• Inventors: HANEDA, Keigo, ENDOH, Takashi
• Granted Patent: US 10,702,503 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 31/4168 having a nitrogen attached in position 2, e.g. clonidine
• CPC Class Codes: C07D 233/52 with hetero atoms directly attached to said nitrogen atoms
• CPC Class Codes: C12N 9/99 Enzyme inactivation by chemical treatment
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/30 involving catalase
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 2333/908 acting on hydrogen peroxide as acceptor (1.11)
• CPC Class Codes: G01N 27/416 Systems G01N27/27 takes precedence
• CPC Class Codes: G01N 33/5008 for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin

Abstract

Provided is a catalase inhibitor comprising a compound represented by formula (I):

wherein R₁ to R₄ independently represent a hydrogen atom, a halogen atom, an amino group, a hydroxyl group, a carbonyl group, or a hydrocarbon group having 1 to 4 carbon atoms, wherein the hydrocarbon group may have at least one substituent selected from the group consisting of a halogen atom, an amino group, a hydroxyl group and a carbonyl group; and X⁻ represents an anionic chemical species.

Claims

1. A catalase inhibitor comprising

a compound represented by formula (I):

2. The catalase inhibitor according to claim 1, wherein

R₁ to R₄ each independently represents a hydrogen atom or a hydrocarbon group having 1 to 4 carbons, wherein the hydrocarbon group may contain at least one kind of substituent selected from the group consisting of a halogen, an amino group, a hydroxyl group, and a carboxyl group;

and

X⁻

represents any one selected from the group consisting of a halogen ion, an ion in a compound containing halogen(s), a hydroxide ion, a carboxylate ion, a nitrate ion, a nitrite ion, an acetate ion, a carbonic acid hydrogen ion, a dihydrogen phosphate ion, a hydrogen sulfate ion, a hydrogen sulfide ion, a hydrogen oxalate ion, a cyanate ion, and a thiocyanate ion.

3. The catalase inhibitor according to claim 1, wherein

R₁ to R₄ each independently represents a hydrogen atom or an unsubstituted, straight-chained or branched-chained saturated hydrocarbon group having 1 to 4 carbons;

and

X⁻

represents a halogen ion or an ion in a compound containing halogen(s).

4. The catalase inhibitor according to claim 1, wherein

the compound represented by the chemical formula (I) is 2-azido-1,3-dimethylimidazolinium hexafluorophosphate.

5. A method for measuring an analyte based on production of hydrogen peroxide, comprising the following steps:

(A) a step of mixing a sample and the catalase inhibitor according to claim 1, wherein the sample contains the analyte and a catalase;

(B) a step of oxidizing the analyte by a first enzyme to produce hydrogen peroxide; and

(C) a step of measuring the hydrogen peroxide.

6. The method for measuring an analyte according to claim 5, wherein

step (B) is performed after step (A) or simultaneously with step (A).

7. A method for measuring an analyte based on production of a hydrogen peroxide, comprising the following steps:

(a) a step of mixing a sample and the catalase inhibitor according to claim 1, wherein the sample contains the analyte and a catalase;

(b-1) a step of producing a substrate from the analyte by a second enzyme;

(b-2) a step of oxidizing the substrate by a first enzyme to produce hydrogen peroxide; and

(c) a step of measuring the hydrogen peroxide.

8. The method for measuring an analyte according to claim 7, wherein

the steps are performed:

in an order of (b-1), (a), (b-2), and then (c);

after step (b-1), steps (a) and (b-2) are performed simultaneously, and then step (c) is performed;

or

steps (a) and (b-1) are performed simultaneously, and then step (c) is performed after step (b-2).

9. The method for measuring an analyte according to claim 5, wherein

in step (C) or (c), the hydrogen peroxide is measured by a colorimetry, a chemiluminescence method, or an electrochemical method.

10. A measurement kit which measures an analyte in a sample containing a catalase, comprising:

a first enzyme which catalyzes a reaction where the analyte is oxidized to produce hydrogen peroxide; and

the catalase inhibitor according to claim 1.

11. A measurement kit that measures an analyte in a sample containing a catalase, comprising:

a first enzyme which catalyzes a reaction where a substrate is oxidized to produce hydrogen peroxide;

the catalase inhibitor according to claim 1; and

a second enzyme which catalyzes a reaction where the substrate is produced from the analyte.

12. The kit according to claim 10, further comprising

a peroxidase.

13. A reagent for pretreatment of a sample for measurement by an electrochemical method, comprising

the catalase inhibitor according to claim 1.

14. A kit for pretreatment of a sample for measurement of an analyte in the sample containing a catalase by an electrochemical method, based on production of hydrogen peroxide by oxidization of a substrate by a first enzyme, wherein said kit for pretreatment of the sample comprises:

the catalase inhibitor according to claim 1; and

a second enzyme which catalyzes a reaction where the substrate is produced from the analyte.