DEVELOPMENT OF AMNION-LIKE TISSUE FROM HUMAN PLURIPOTENT STEM CELLS

• US 20190321415A1
• Filed: 12/08/2017
• Published: 10/24/2019
• Est. Priority Date: 12/09/2016
• Status: Active Grant

• Current Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Original Assignee: Board of Regents of the University of Michigan (University of Michigan)
• Inventors: Fu, Jianping, Gumucio, Deborah, Shao, Yue, Taniguchi, Kenichiro, Zheng, Yi, Esfahani, Sajedeh Nasr
• Granted Patent: US 11,672,832 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 31/74 Synthetic polymeric materials
• CPC Class Codes: A61K 35/50 Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
• CPC Class Codes: A61K 35/545 Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
• CPC Class Codes: C12N 2502/45 Artificially induced pluripotent stem cells
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0605 Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
• CPC Class Codes: G01N 33/5044 involving specific cell types

Abstract

The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Claims

1. A method for preparing amnion-like tissue, comprising:

culturing cells on a solid support coated with a gel matrix, wherein said cells are coated with further gel matrix under conditions such that amnion-like tissue is generated.

2. The method of claim 1, wherein said cells are stem cells.

3. The method of claim 1, wherein said cells are induced pluripotent cells.

4. The method of claim 1, wherein said solid support comprises a plurality of microposts.

5. The method of claim 2, wherein the stem cells are induced pluripotent stem cells (iPSCs).

6. The method of claim 5, wherein the iPSCs are human iPSCs.

7. The method of claim 2, wherein the stem cells are human embryonic stem cells.

8. The method of claim 1, wherein said surface is glass or PDMS.

9. The method of claim 1, wherein said gel matrix is a natural or synthetic polymeric hydrogel.

10. The method of claim 9, wherein said gel matrix is growth factor basement membrane matrix or Matrigel.

11. The method of claim 1, wherein said amnion-like cells are an asymmetric cyst.

12. The method of claim 1, wherein said cells are generated in a device comprising parallel first, second, and third channels wherein said first and second channels are cell channels comprising a loading reservoir operably linked to said third channel comprising a gel interaction matrix.

13. A composition comprising a plurality of cells produced by the method of claim 1.

14. A method for testing a compound, comprising:

a) providing a composition of claim 13;

b) exposing a test compound to said composition; and

c) determining an effect of said test compound on said composition.