METHOD OF BONE REGENERATION

• US 20190321517A1
• Filed: 04/12/2019
• Published: 10/24/2019
• Est. Priority Date: 04/19/2018
• Status: Active Grant

• Current Assignee: Paulo Jose Pasquali
• Original Assignee: Paulo Jose Pasquali
• Inventors: PASQUALI, Paulo Jose
• Granted Patent: US 11,491,262 B2
• Time in Patent Office: Days
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/2803 for mandibular reconstruction temporo-mandibular joints A61F2/3099; bone plates for the jaw A61B17/8071
• CPC Class Codes: A61F 2002/2835 Bone graft implants for filling a bony defect or an endoprosthesis cavity, e.g. by synthetic material or biological material
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61L 2300/412 Tissue-regenerating or healing or proliferative agents
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 27/3608 Bone, e.g. demineralised bone matrix [DBM], bone powder
• CPC Class Codes: A61L 27/3616 Blood, e.g. platelet-rich plasma
• CPC Class Codes: A61L 27/3821 Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
• CPC Class Codes: A61L 27/3847 Bones

Abstract

The present invention provides a method of bone regeneration for repairing a bone defect in a subject in need thereof. The method comprises the use of blood aspirate of the mandible bone marrow with the use of xenogen bone support.

Claims

1. A method of bone regeneration for repairing a bone defect in a subject in need thereof, wherein the method comprises the steps of:

exposing a bone repair site in the subject, wherein the bone repair site contains a bone having a bone defect;

fixing a graft containing bone-tissue-forming cells in the receptor bed of the bone;

collecting bone marrow blood from the mandible of the subject being treated;

applying the collected mandibular bone marrow blood to the graft; and

placing a layer of barrier material over at least a portion of the graft.

2. The method according to claim 1, wherein the bone defect is a lost or atrophied bone tissue.

3. The method according to claim 1, wherein the bone repair site is an orthopedic, periodontal, neurosurgical, oral or maxillofacial bone repair site.

4. The method according to claim 3, wherein the bone repair site is a periodontal bone repair site.

5. The method according to claim 1, wherein the barrier is a non-absorbable biocompatible barrier.

6. The method according to claim 5, wherein the barrier is a polypropylene barrier.

7. The method according to claim 1, wherein the graft is a bone graft.

8. The method according to claim 7, wherein the bone graft is of autogenous, allogenic or xenogeneic origin.

9. The method according to claim 8, wherein the bone graft is of xenogeneic origin.

10. The method according to claim 9, wherein the bone graft is obtained from porcine or bovine bone.

11. The method according to claim 1, wherein the graft is in the form of a block, particulate or assembly.

12. The method according to claim 1, wherein the receptor bed is the remaining bone base in the bone.

13. The method according to claim 1, wherein the subject is a mammal.

14. The method according to claim 13, wherein the subject is human.

15. The method according to claim 1, wherein the exposing step comprises treating the bone repair site with local anesthesia and/or providing gingival clearance.

16. The method according to claim 1, wherein the collecting step comprises aspirating the bone marrow blood from the mandible.

17. The method according to claim 1, wherein the applying step comprises humidifying the graph with the bone marrow blood.

18. The method according to claim 1, wherein the graft is a block graft and wherein the block is a surgical implant.

19. The method according to claim 18, wherein the implant is a dental implant.