SYNTHESIS OF GLUCAN COMPRISING BETA-1,3 GLYCOSIDIC LINKAGES WITH PHOSPHORYLASE ENZYMES
First Claim
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1. A reaction composition comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, or SEQ ID NO;
12, wherein the enzyme synthesizes beta-1,3-glucan.
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Abstract
Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.
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Citations
18 Claims
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1. A reaction composition comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, or SEQ ID NO;
12, wherein the enzyme synthesizes beta-1,3-glucan. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
- 2, SEQ ID NO;
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11. A method for producing beta-1,3-glucan, said method comprising:
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(a) contacting at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO;
2, SEQ ID NO;
4, SEQ ID NO;
6, SEQ ID NO;
8, SEQ ID NO;
10, or SEQ ID NO;
12, wherein beta-1,3-glucan is produced; and(b) optionally, isolating the beta-1,3-glucan produced in step (a). - View Dependent Claims (12, 13, 14, 15, 16)
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17. A method of processing beta-1,3-glucan, said method comprising:
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(a) providing an aqueous composition comprising up to about 40 wt % insoluble beta-1,3-glucan having a degree of polymerization (DP) of at least 17; (b) heating the aqueous composition to at least about 75°
C., thereby dissolving the beta-1,3-glucan in the aqueous composition to provide a solution;(c) subjecting the solution to at least one process that reduces the content of one or more solutes other than the dissolved beta-1,3-glucan, thereby increasing the content of the dissolved beta-1,3-glucan in the solution on a dry weight basis; and (d) optionally cooling the solution to a temperature at which the beta-1,3-glucan precipitates back to an insoluble state, and optionally isolating the precipitated beta-1,3-glucan. - View Dependent Claims (18)
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Specification