METHOD FOR FERMENTATION-PRODUCTION OF PENTANEDIAMINE COMPRISING CARBON DIOXIDE STRIPPING TECHNIQUE

• US 20190322995A1
• Filed: 05/16/2017
• Published: 10/24/2019
• Est. Priority Date: 05/16/2016
• Status: Active Grant

• Current Assignee: Heilongjiang Eppen New Materials, Co., Ltd.
• Original Assignee: Ningxia Eppen Biotech Co. Ltd (Star Lake Bioscience Company Incorporated Zhaoqing Guangdong)
• Inventors: MA, Jiyin, WEN, Tingyi, LIU, Shuwen, LIANG, Yong, LI, Daihuan, ZHANG, Yun, SHANG, Xiuling, ZHAO, Chunguang, GUO, Xiaowei, MENG, Gang
• Granted Patent: US 11,060,080 B2
• Time in Patent Office: Days
• CPC Class Codes: B01D 1/18 to obtain dry solids B01D1/24 takes precedence
• CPC Class Codes: B01D 19/00 Degasification of liquids
• CPC Class Codes: B01D 19/0036 Flash degasification the other groups take precedence
• CPC Class Codes: B01D 19/0073 by a method not covered by groups B01D19/0005 - B01D19/0042
• CPC Class Codes: B01D 3/06 Flash distillation
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: C12P 1/00 Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
• CPC Class Codes: C12P 1/04 by using bacteria
• CPC Class Codes: C12P 13/00 Preparation of nitrogen-containing organic compounds
• CPC Class Codes: C12P 13/001 Amines; Imines
• CPC Class Codes: C12R 2001/19 Escherichia coli
• CPC Class Codes: C12Y 401/01018 Lysine decarboxylase (4.1.1.18)

Abstract

A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.

Claims

1. A method for preparing 1,5-pentanediamine by fermentation, comprising:

(1) culturing a cell expressing a lysine decarboxylase to obtain a fermentation broth comprising 1, 5-pentanediamine;

(21) separating the liquid from the fermentation broth;

(22) removing carbon dioxide from the liquid obtained in the step (21), and then adding a base for treatment;

(23) extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (22).

2. The method according to claim 1, wherein in step (1), the cell is a cell overexpressing lysine decarboxylase.

3. The method according to claim 1, wherein in step (1), the cell is a cell with enhanced lysine decarboxylase gene expression.

4. The method according to claim 1, wherein in step (1), the cell is a cell whose expression is enhanced by replacing the promoter of the lysine decarboxylase gene with a strong promoter.

5. The method according to claim 1, wherein in step (1), the cell is a cell whose expression is enhanced by replacing the promoter of the lysine decarboxylase gene with T7 promoter.

6. The method according to claim 1, wherein in step (1), the cell is a bacterial cell.

7. The method according to claim 1, wherein in step (1), the cell is an E. coli cell.

8. The method according to claim 1, wherein in step (1), the cell is E. coli BL21 (DE3).

9. The method according to claim 1, wherein in step (1), no acidic pH regulator is added to the culture, and the cells themselves generate CO₂ to neutralize 1,5-pentanediamine.

10. The method according to claim 1, wherein in step (1), the bacteria produce 1,5-pentadiamine under catalyzation by lysine decarboxylase with lysine as a substrate.

11. The method according to claim 1, wherein in step (21), the separating is centrifuging or filtering.

12. The method according to claim 1, wherein in step (22), carbon dioxide in the liquid is removed by decompressing and/or heating to separate the carbon dioxide in the liquid.

13. The method according to claim 1, wherein in step (22), the base includes sodium hydroxide, potassium hydroxide and/or calcium hydroxide.

14. The method according to claim 1, wherein in step (23), the composition is a fraction.

15. The method according to claim 1, wherein in step (23), the extracting includes distilling, evaporating and/or drying.

16. The method according to claim 15, wherein in step (23), the distilling is atmospheric distilling, reduced pressure distilling and/or rectifying.

17. The method according to claim 15, wherein in step (23), the evaporating is flash evaporating.

18. The method according to claim 15, wherein in step (23), the drying is spray drying and/or reduced pressure drying.

19. The method according to claim 1, wherein the output of 1,5-pentanediamine is greater than 30 g/L of the fermentation broth.

20. The method according to claim 19, wherein the output of 1,5-pentanediamine is greater than 50 g/L of the fermentation broth.

21. The method according to claim 20, wherein the output of 1,5-pentanediamine is greater than 80 g/L of the fermentation broth.

22. The method according to claim 21, wherein the output of 1,5-pentanediamine is greater than 100 g/L of the fermentation broth.

23. The method according to claim 22, wherein the output of 1,5-pentanediamine is greater than 120 g/L of the fermentation broth.