METHOD FOR FERMENTATION-PRODUCTION OF PENTANEDIAMINE COMPRISING CARBON DIOXIDE STRIPPING TECHNIQUE
First Claim
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1. A method for preparing 1,5-pentanediamine by fermentation, comprising:
- (1) culturing a cell expressing a lysine decarboxylase to obtain a fermentation broth comprising 1, 5-pentanediamine;
(21) separating the liquid from the fermentation broth;
(22) removing carbon dioxide from the liquid obtained in the step (21), and then adding a base for treatment;
(23) extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (22).
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Abstract
A method for fermentation-production of a pentanediamine, comprising: culturing a cell expressing a lysine decarboxylase to obtain a whole cell fermentation broth comprising a pentanediamine; and extracting the pentanediamine from the whole cell fermentation broth, and striping the whole cell fermentation broth of carbon dioxide contained therein before adding a strong base. The method greatly increases a production volume of the pentanediamine.
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Citations
23 Claims
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1. A method for preparing 1,5-pentanediamine by fermentation, comprising:
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(1) culturing a cell expressing a lysine decarboxylase to obtain a fermentation broth comprising 1, 5-pentanediamine; (21) separating the liquid from the fermentation broth; (22) removing carbon dioxide from the liquid obtained in the step (21), and then adding a base for treatment; (23) extracting a composition containing 1,5-pentanediamine from the treatment liquid obtained in the step (22). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification