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METHOD OF PRODUCING DNA AND DNA LIBRARY

  • US 20190323005A1
  • Filed: 04/19/2018
  • Published: 10/24/2019
  • Est. Priority Date: 04/19/2018
  • Status: Active Grant
First Claim
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1. A method of producing a cDNA from a sample of nucleic acids, comprising the steps of:

  • a) generating a dephosphorylated RNA from the sample;

    b) ligating the dephosphorylated RNA with a first adapter in the presence of a crowding agent to produce a ligated product;

    c) removing the excess first adapter by adding at least two enzymes; and

    d) performing reverse transcription in a lithium-containing buffer to produce the cDNA.

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