COMPOSITIONS AND METHODS FOR DELIVERY OF MOLECULES TO PLANTS
First Claim
1. A method of producing a plant comprising an avenic acid transporter and having timed senescence, said method comprising introducing in said plant,a) an avenic acid transporter andb) one or more constructs comprising:
- (i) a floral promoter or an inducible promoter, or both; and
(ii) nucleic acid molecules expressing heavy and lights chains forming an antibody specific to said avenic acid transporter and/or a functional small peptide; and
c) producing a plant having senescence timed with expression of said floral promoter and/or exposure to inducing conditions of said inducible promoter.
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Abstract
Compositions and methods are provided where a plant comprising an avenic acid transporter is planted in a field with a target plant in need of increased iron uptake. The plant comprising the avenic acid transporter in an embodiment further comprises one or more constructs comprising nucleic acid molecules expressing heavy and light chains forming an antibody specific to the avenic acid transporter or functional small peptide and may also include a sequence targeting the apoplast. The antibody or functional small peptide is operably linked to a floral promoter or inducible natural or synthetic promoter. Upon expression regulated by the floral promoter or exposure to the inducing conditions, or both, the plant increases its production of avenic acid transporter antibodies, enters senescence and eventually dies. As a result, the plant is not harvested with the target plant, provides a cover crop, and does not continue to use nutrients, and other resources that benefit the targeted plant. Increased avenic acid is produced by the plant prior to dying in response to Fe3+ starvation induced by inactivation of the Fe+3/phytosiderophore transporter.
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20 Claims
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1. A method of producing a plant comprising an avenic acid transporter and having timed senescence, said method comprising introducing in said plant,
a) an avenic acid transporter and b) one or more constructs comprising: -
(i) a floral promoter or an inducible promoter, or both; and (ii) nucleic acid molecules expressing heavy and lights chains forming an antibody specific to said avenic acid transporter and/or a functional small peptide; and c) producing a plant having senescence timed with expression of said floral promoter and/or exposure to inducing conditions of said inducible promoter. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A plant comprising one or more constructs comprising,
a) a floral promoter or an inducible promoter, or both; - and
b) nucleic acid molecules expressing heavy and lights chains forming an antibody specific to an avenic acid transporter and/or functional small peptide. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A method of producing senescence in nurse plants planted in a field with target plants, comprising:
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a) planting a first plurality of target plants; b) planting a second plurality of nurse plants comprising, (i) an avenic acid transporter; and (ii) one or more constructs comprising; (a) a floral promoter or an inducible promoter, or both; (b) nucleic acid molecules expressing heavy and lights chains forming an antibody specific to said avenic acid transporter and/or a functional small peptide; and c) causing senescence of said nurse plants upon expression of said floral tissue preferred promoter and/or upon exposure to inducing conditions of said inducible promoter. - View Dependent Claims (14, 15, 16, 17, 18, 19)
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20. A method of providing iron to a target plant, the method comprising,
a) planting a first plurality of target plants; -
b) planting a second plurality of nurse plants comprising, (i) an avenic acid transporter and (ii) one or more constructs comprising; (a) a floral promoter or an inducible promoter, or both; (b) heavy and lights chains forming an antibody specific to said avenic acid transporter; and c) causing senescence of said nurse plants upon expression of said floral tissue preferred promoter and/or upon exposure to inducing conditions of said inducible promoter, wherein said nurse plants produced increased amounts of avenic acid transporter upon binding of said antibody to said avenic acid transporter.
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Specification