KETOREDUCTASE POLYPEPTIDES FOR THE PRODUCTION OF (R)-3-HYDROXYTHIOLANE

• US 20190323044A1
• Filed: 06/28/2019
• Published: 10/24/2019
• Est. Priority Date: 08/24/2007
• Status: Active Grant

• Current Assignee: Codexis, Inc.
• Original Assignee: Codexis, Inc.
• Inventors: Liang, Jack S, Jenne, Stephan, Mundorff, Emily, Voladri, Rama, Lalonde, James J., Huisman, Gjalt W.
• Granted Patent: US 10,851,397 B2
• Time in Patent Office: Days
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)
• CPC Class Codes: C12P 17/00 Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms C12P13/04 - C12P13/24 take precedence
• CPC Class Codes: C12P 17/167 Heterorings having sulfur atoms as ring heteroatoms, e.g. vitamin B1, thiamine nucleus and open chain analogs
• CPC Class Codes: C12Y 101/01184 Carbonyl reductase (NADPH) (1.1.1.184)
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize chiral compounds.

Claims

1. An engineered polynucleotide encoding an engineered ketoreductase polypeptide, wherein the engineered ketoreductase polypeptide comprises an amino acid sequence that is at least 90% identical to the reference sequence SEQ ID NO:

4, and wherein said amino acid sequence comprises a valine or threonine at position 94.

2. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises one or more additional substitutions selected from:

the residue corresponding to X3 is asparagine, aspartic acid, or tyrosine;

the residue corresponding to X7 is glycine, histidine, serine or asparagine;

the residue corresponding to X11 is isoleucine or threonine;

the residue corresponding to X16 is threonine, alanine, valine, or glycine;

the residue corresponding to X19 is isoleucine or valine;

the residue corresponding to X23 is isoleucine or phenylalanine;

the residue corresponding to X41 is serine, alanine, or valine;

the residue corresponding to X45 is glutamic acid or glycine;

the residue corresponding to X49 is lysine or arginine;

the residue corresponding to X57 is isoleucine or valine;

the residue corresponding to X60 is phenylalanine, valine, or threonine;

the residue corresponding to X64 is alanine, serine, or threonine;

the residue corresponding to X72 is lysine or arginine, particularly arginine;

the residue corresponding to X82 is glycine or serine;

the residue corresponding to X95 is valine or alanine;

the residue corresponding to X96 is asparagine, serine, proline, alanine, or glutamic acid;

the residue corresponding to X97 is lysine, arginine or leucine;

the residue corresponding to X106 is glutamic acid or aspartic acid;

the residue corresponding to X108 is arginine or histidine;

the residue corresponding to X111 is leucine or methionine;

the residue corresponding to X117 is glycine or serine;

the residue corresponding to X126 is isoleucine or valine;

the residue corresponding to X127 is glutamine or arginine;

the residue corresponding to X145 is aspartic acid or serine;

the residue corresponding to X147 is phenylalanine, leucine or serine;

the residue corresponding to X152 is threonine, serine, or methionine;

the residue corresponding to X157 is asparagine, glutamine, threonine, serine, or aspartic acid;

the residue corresponding to X163 is valine or isoleucine;

the residue corresponding to X173 is aspartic acid or glycine;

the residue corresponding to X177 is lysine or arginine;

the residue corresponding to X192 is lysine, arginine or glutamic acid;

the residue corresponding to X194 is proline, glycine, aspartic acid, arginine, or leucine;

the residue corresponding to X198 is aspartic acid or glycine;

the residue corresponding to X200 is proline, glutamic acid, or lysine;

the residue corresponding to X206 is methionine or glutamine;

the residue corresponding to X208 is glutamine, histidine or arginine;

the residue corresponding to X210 is threonine or alanine;

the residue corresponding to X211 is lysine or glutamic acid;

the residue corresponding to X214 is methionine, valine or threonine, or serine;

the residue corresponding to X217 is isoleucine or phenylalanine;

the residue corresponding to X223 is isoleucine or valine; and

the residue corresponding to X226 is isoleucine or valine.

3. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, wherein the ketoreductase polypeptide amino acid sequence further comprises at least one additional substitution selected from:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X96 is a constrained, aliphatic, non-polar, acidic, or polar residue;

the residue corresponding to X108 is a basic, constrained, or aromatic residue;

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X147 is a non-polar or alphatic residue;

the residue corresponding to X157 is a polar or acidic residue;

the residue corresponding to X173 is an acidic or non-polar residue;

the residue corresponding to X206 is a polar or non-polar residue; and

the residue corresponding to X223 is an aliphatic or non-polar residue.

4. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises the following substitutions:

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X147 is a non-polar or alphatic residue;

the residue corresponding to X157 is a polar or acidic residue.

5. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises at least one substitution selected from:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X108 is a basic, constrained, or aromatic residue;

the residue corresponding to X117 is a non-polar or polar residue, particularly serine;

the residue corresponding to X147 is a non-polar or alphatic residue;

the residue corresponding to X157 is a polar or acidic residue, particularly a threonine.

6. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence comprises the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X96 is a constrained, aliphatic, non-polar, acidic, or polar residue;

the residue corresponding to X108 is a basic, constrained, or aromatic residue;

the residue corresponding to X117 is a non-polar or polar residue, particularly serine;

the residue corresponding to X147 is leucine; and

the residue corresponding to X157 is a polar or acidic residue, particularly threonine.

7. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X96 is a constrained, aliphatic, non-polar, acidic, or polar residue;

residue corresponding to X108 is a basic, constrained, or constrained residue;

the residue corresponding to X117 is a non-polar or polar residue, particularly serine;

the residue corresponding to X157 is a polar or acidic residue, particularly a threonine.

8. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X108 is a basic, constrained, or aromatic residue;

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X157 is a polar or acidic residue; and

the residue corresponding to X206 is a polar or non-polar residue.

9. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises at least one of the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X96 is a constrained, aliphatic, non-polar, acidic, or polar residue;

the residue corresponding to X108 is a basic, constrained, or aromatic residue;

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X157 is a polar or acidic residue; and

the residue corresponding to X206 is a polar or non-polar residue.

10. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises the following substitutions:

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X157 is a polar or acidic residue;

the residue corresponding to X173 is an acidic or non-polar residue; and

the residue corresponding to X206 is a polar or non-polar residue.

11. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X157 is a polar or acidic residue;

the residue corresponding to X173 is an acidic or non-polar residue; and

the residue corresponding to X206 is a polar or non-polar residue.

12. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, in which the ketoreductase polypeptide amino acid sequence further comprises at least one of the following substitutions:

the residue corresponding to X7 is a non-polar, polar, or constrained residue;

the residue corresponding to X96 is a constrained, aliphatic, non-polar, acidic, or polar residue;

the residue corresponding to X117 is a non-polar or polar residue;

the residue corresponding to X157 is a polar or acidic residue;

the residue corresponding to X173 is an acidic or non-polar residue; and

the residue corresponding to X206 is a polar or non-polar residue.

13. The engineered polynucleotide encoding the engineered ketoreductase polypeptide of claim 1, wherein the engineered ketoreductase polypeptide produces product compounds with percent stereomeric excess of at least 90%.

14. The engineered polynucleotide of claim 1, wherein the polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:

5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, and 133.

16. An expression vector comprising the engineered polynucleotide of claim 1.

17. The expression vector of claim 16, further comprising at least one control sequence.

18. A host cell comprising the engineered polynucleotide of claim 1.

19. A host cell comprising the expression vector of claim 16.

20. A host cell comprising the expression vector of claim 17.

15. An engineered polynucleotide encoding an engineered ketoreductase polypeptide, wherein the polynucleotide comprises a sequence that is at least 90% identical to a sequence selected from the group consisting of SEQ ID NO:

5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, 95, 97, 99, 101, 103, 105, 107, 109, 111, 113, 115, 117, 119, 121, 123, 125, 127, 129, 131, and 133.