SUBSTRATE WITH MATRIX-FREE NANOSTRUCTURED HYDROPHILIC ANALYTE SPOTS FOR USE IN MASS SPECTROMETRY
First Claim
1. A substrate configured to receive one or more samples for analysis using laser desorption/ionization mass spectrometry comprising:
- a. one or more localized analyte spots; and
b. a substrate base;
wherein the one or more analyte spots comprise primary nanostructures comprising at least one nanoporous or nanotubular metal oxide or semiconductor;
wherein the one or more analyte spots have a diameter of between about 0.1 mm and 10 mm;
wherein the substrate base comprises a non-nanoporous and non-nanotubular metal, metal oxide, or semiconductor; and
wherein the one or more analyte spots are more hydrophilic than the substrate base.
0 Assignments
0 Petitions
Accused Products
Abstract
The present disclosure describes a matrix-free nanostructured substrate for use in mass spectrometry. The substrate may preferably include one or more localized analyte spots for placement of an analyte, where each analyte spot may comprise a nanostructured metal oxide or semiconductor containing nanotubes or nanopores. The substrate may further include unstructured metal, metal oxide, or semiconductor that is not nanotubular or nanoporous in the part of the substrate that surrounds each of the analyte spots. In some embodiments, the nanostructured metal oxide or semiconductor may be chemically or structurally modified, and the analyte spots may additionally or alternatively include secondary nanostructures such as nanorods, nanoparticles, nanocoatings, or nanotubes. This may facilitate energy transfer to the analyte for matrix-free laser desorption/ionization. The analyte spots may preferably be more hydrophilic than the surrounding part of the substrate to ensure concentration of the analyte at the analyte spots.
0 Citations
20 Claims
-
1. A substrate configured to receive one or more samples for analysis using laser desorption/ionization mass spectrometry comprising:
-
a. one or more localized analyte spots; and b. a substrate base; wherein the one or more analyte spots comprise primary nanostructures comprising at least one nanoporous or nanotubular metal oxide or semiconductor; wherein the one or more analyte spots have a diameter of between about 0.1 mm and 10 mm; wherein the substrate base comprises a non-nanoporous and non-nanotubular metal, metal oxide, or semiconductor; and wherein the one or more analyte spots are more hydrophilic than the substrate base. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification