COMBINATION PRODUCT FOR DETECTING TARGET MARKER

• US 20190324020A1
• Filed: 04/30/2019
• Published: 10/24/2019
• Est. Priority Date: 04/23/2014
• Status: Active Grant

• Current Assignee: Nichirei Biosciences Inc. (Nichirei Corporation)
• Original Assignee: Nichirei Biosciences Inc. (Nichirei Corporation)
• Inventors: OHBAYASHI, Hirokazu, FUJITA, Kayoko
• Granted Patent: US 11,156,602 B2
• Time in Patent Office: Days
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/42 involving phosphatase
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: G01N 33/5306 Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
• CPC Class Codes: G01N 33/532 Production of labelled immunochemicals
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: G01N 33/536 with immune complex formed in liquid phase
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/581 with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
• CPC Class Codes: G01N 33/6857 Antibody fragments

Abstract

The present invention relates to a combination product for detecting a target marker simply and with high sensitivity. More specifically, the present invention relates to a combination product for detecting a target marker in a biological sample in combination with a target marker binding molecule which is capable of binding specifically to the target marker in the biological sample, the combination comprising, at least:

• • (a) a first binding agent comprising a first binding molecule which is capable of directly or indirectly binding specifically to the target marker binding molecule, and a labeling substance;
  • (b) a linker molecule which is capable of binding specifically to the first binding agent; and
  • (c) a second binding agent which is capable of binding specifically to the linker molecule, and comprises a second binding molecule and a labeling substance.

Claims

1-20. -20. (canceled)

21. A combination product for detecting a target marker in a biological sample in combination with a target marker binding molecule which is capable of binding specifically to the target marker, the combination product comprising, at least:

(a) a first binding agent comprising a first binding molecule which is capable of directly or indirectly binding specifically to the target marker binding molecule, and a labeling substance;

(b) a linker molecule which is capable of binding specifically to the first binding agent; and

(c) a second binding agent which is capable of binding specifically to the linker molecule, and comprises a second binding molecule and a labeling substance.

22. The combination product according to claim 21, wherein the linker molecule is capable of binding specifically to the labeling substance.

23. The combination product according to claim 21, wherein the linker molecule is an antibody or an antigen binding fragment thereof.

24. The combination product according to claim 21, wherein the labeling substance is at least one selected from a chemiluminescent label, a metal particle, a fluorescent label, an enzyme label, a coenzyme label, a labeled antibody, a dye, a bioluminescent label, a hapten and a polymer particle.

25. The combination product according to claim 21, wherein the first binding agent is a structure in which the first binding molecule and the labeling substance are connected with each other directly or indirectly via a carrier.

26. The combination product according to claim 21, wherein the second binding agent is a structure in which the second binding molecule and the labeling substance are connected with each other directly or indirectly via a carrier.

27. The combination product according to claim 21, wherein the first binding molecule is an antibody or an antigen binding fragment thereof.

28. The combination product according to claim 21, wherein the second binding molecule is an antibody or an antigen binding fragment thereof.

29. The combination product according to claim 21, which is further combined with the target marker binding molecule.

30. The combination product according to claim 21, which is in the form of a kit.