CRYOPRESERVATION VESSEL

• US 20190327959A1
• Filed: 06/09/2017
• Published: 10/31/2019
• Est. Priority Date: 06/15/2016
• Status: Active Grant

• Current Assignee: Nissan Chemical Corporation
• Original Assignee: Nissan Chemical Corporation
• Inventors: HIROI, Yoshiomi, NAKAJIMA, Hiroyuki, NISHINO, Taito
• Granted Patent: US 11,470,841 B2
• Time in Patent Office: Days
• CPC Class Codes: A01N 1/0268 Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
• CPC Class Codes: C08F 220/34 Esters containing nitrogen , e.g. N,N-dimethylaminoethyl (meth)acrylate
• CPC Class Codes: C08F 230/02 containing phosphorus
• CPC Class Codes: C09D 143/02 Homopolymers or copolymers of monomers containing phosphorus
• CPC Class Codes: C12M 3/00 Tissue, human, animal or plant cell, or virus culture apparatus

Abstract

An object of the present invention is to provide a cryopreservation vessel which has a coating that is capable of inhibiting the adhesion of cells or proteins and is resistant not only to aqueous solvents but also to organic solvents (for example, cryoprotectants, in particular, dimethyl sulfoxide). The invention provides a cell or protein cryopreservation vessel including, on at least a portion of the surface thereof, a coating that includes a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b) (wherein U^a1, U^a2, U^a3, U^b1, U^b2, U^b3 and An⁻ are as defined in the description and the claims)

Claims

1. A cell or protein cryopreservation vessel comprising a coating on at least a portion of the surface thereof, wherein the coating comprises a copolymer including a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b):

2. The vessel according to claim 1, wherein the copolymer further includes a repeating unit containing a group represented by the following formula (c):

—

R^c 

 

(c)

wherein

R^c is a C_1-8s linear or branched alkyl group, a C_3-10 cyclic hydrocarbon group, a C_6-10 aryl group, a C_7-15 aralkyl group or a C_7-15 aryloxyalkyl group, wherein the aryl moiety may be substituted with a C_1-5 linear or branched alkyl group which may be substituted with a halogen atom.

3. The vessel according to claim 1, wherein the copolymer includes repeating units represented by the following formulae (a1) and (b1):

4. The cryopreservation vessel according to claim 2, wherein the copolymer further includes a repeating unit represented by the following formula (c1):

5. The vessel according to claim 1, wherein the coating has a resistance to a cryoprotectant.

6. The vessel according to claim 5, wherein the cryoprotectant is selected from the group consisting of ethylene glycol, propanediol, methanol, ethanol, dimethylacetamide, glycerol, polyethylene glycol, polyvinylpyrrolidone, hydroxyethyl starch, dextran, albumin, acetamide, acrylamide, propionamide, methacrylamide, isobutyramide, lactamide, nicotinamide, isophthalamide, acetonitrile, acetone and dimethyl sulfoxide.

7. The vessel according to claim 2, wherein the coating has a resistance to a cryoprotectant.

8. The vessel according to claim 7, wherein the cryoprotectant is selected from the group consisting of ethylene glycol, propanediol, methanol, ethanol, dimethylacetamide, glycerol, polyethylene glycol, polyvinylpyrrolidone, hydroxyethyl starch, dextran, albumin, acetamide, acrylamide, propionamide, methacrylamide, isobutyramide, lactamide, nicotinamide, isophthalamide, acetonitrile, acetone and dimethyl sulfoxide.

9. The vessel according to claim 3, wherein the coating has a resistance to a cryoprotectant.

10. The vessel according to claim 9, wherein the cryoprotectant is selected from the group consisting of ethylene glycol, propanediol, methanol, ethanol, dimethylacetamide, glycerol, polyethylene glycol, polyvinylpyrrolidone, hydroxyethyl starch, dextran, albumin, acetamide, acrylamide, propionamide, methacrylamide, isobutyramide, lactamide, nicotinamide, isophthalamide, acetonitrile, acetone and dimethyl sulfoxide.

11. The vessel according to claim 4, wherein the coating has a resistance to a cryoprotectant.

12. The vessel according to claim 11, wherein the cryoprotectant is selected from the group consisting of ethylene glycol, propanediol, methanol, ethanol, dimethylacetamide, glycerol, polyethylene glycol, polyvinylpyrrolidone, hydroxyethyl starch, dextran, albumin, acetamide, acrylamide, propionamide, methacrylamide, isobutyramide, lactamide, nicotinamide, isophthalamide, acetonitrile, acetone and dimethyl sulfoxide.