MESENCHYMAL STEM CELL-DERIVED EXTRACELLULAR VESICLES AND THEIR MEDICAL USE
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Abstract
The present invention relates to a method for obtaining extracellular vesicles derived from mesenchymal stromal cells (MSCs), wherein the extracellular-vesicles are essentially free of vesicles not derived from the MSCs, and wherein the extracellular-vesicles are essentially free of heparin. The invention further relates to the extracellular vesicles derived from mesenchymal stromal cells (MSCs), to a pharmaceutical composition comprising such extracellular-vesicles and to the use of the pharmaceutical composition in the treatment of (a) bone defect(s), tendon defect(s) and/or spinal cord injury. The pharmaceutical composition of the invention can further comprise one or more Bone Morphogenic Proteins (BMPs), such as BMP2.
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Citations
153 Claims
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1-53. -53. (canceled)
- 54. Pharmaceutical composition comprising extracellular-vesicles derived from mesenchymal stromal cells (MSCs), wherein the extracellular-vesicles are essentially free of vesicles not derived from the MSCs, and wherein the extracellular-vesicles are essentially free of heparin.
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64. The pharmaceutical composition of 63, wherein the dose of the BMP is about 375 μ
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- 86. A method of treating a bone defect, a tendon defect or a spinal cord injury, wherein the method comprises the administration of a pharmaceutical composition comprising extracellular-vesicles derived from mesenchymal stromal cells (MSCs), wherein the extracellular-vesicles are essentially free of vesicles not derived from the MSCs, and wherein the extracellular-vesicles are essentially free of heparin.
- 118. Extracellular-vesicles derived from mesenchymal stromal cells (MSCs), wherein the extracellular-vesicles are essentially free of vesicles not derived from the MSCs, and wherein the extracellular-vesicles are essentially free of heparin.
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140. A method for obtaining extracellular-vesicles derived from mesenchymal stromal cells (MSCs) that are essentially free of vesicles not derived from the MSCs, wherein the method comprises:
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culturing the MSCs in a culture medium that is essentially free of vesicles not derived from the MSCs and that is essentially free of heparin and preferably fibrinogen; and (ii) isolating the extracellular-vesicles derived from the MSCs from the culture medium. - View Dependent Claims (141, 142, 143)
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144. A method for obtaining extracellular-vesicles derived from mesenchymal stromal cells (MSCs), wherein the method comprises:
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(i) removing fibrinogen from a culture medium, wherein no heparin is added to the culture medium, and removing vesicles from the culture medium, (ii) culturing the MSCs in the culture medium of step (i), and (iii) isolating the extracellular-vesicles derived from the MSCs from the medium. - View Dependent Claims (145, 146, 147, 148, 149)
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- 150. Mesenchymal stromal cells (MSCs) comprised and/or cultured in a culture medium that is essentially free of vesicles not derived from the MSCs, and preferably wherein the culture medium is essentially free of heparin and preferably fibrinogen.
- 152. A culture medium, wherein the culture medium comprises extracellular-vesicles derived from mesenchymal stromal cells (MSCs), and wherein the extracellular-vesicles are essentially free of vesicles not derived from the MSCs, and preferably wherein the culture medium is essentially free of heparin and preferably fibrinogen.
Specification