CHINESE MEDICINAL COMPOSITION FOR PREVENTING OR TREATING CARDIOVASCULAR AND CEREBROVASCULAR DISEASES OR DEMENTIA, AND PREPARATION METHOD AND USE THEREOF

• US 20190328810A1
• Filed: 07/09/2019
• Published: 10/31/2019
• Est. Priority Date: 12/30/2014
• Status: Active Grant

• Current Assignee: Shineway Pharmaceutical Group Ltd.
• Original Assignee: Shineway Pharmaceutical Group Ltd.
• Inventors: Liu, Jianxun, Zhang, Vivian, Li, Zhigang, Zhang, Shyflysky, Guan, Xiuwei, Lu, Iris, Gao, Weibo, Xu, Li, Song, Wenting
• Granted Patent: US 10,967,023 B2
• Time in Patent Office: Days
• CPC Class Codes: A61K 2236/00 Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 36/16 Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
• CPC Class Codes: A61K 36/258 Panax (ginseng)
• CPC Class Codes: A61K 36/88 Liliopsida (monocotyledons)
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis

Abstract

Disclosed are a Chinese medicinal composition for preventing or treating cardiovascular and cerebrovascular diseases and/or dementia, and a preparation method and use thereof. The composition is prepared from the following medical raw materials in parts by weight: 1 part of ginseng, 0.8-1.5 parts of ginkgo leaf and 0.018-0.030 part of stigma croci.

Claims

1. A method of preparing the Chinese medicinal composition, comprising a step of weighing ginseng, ginkgo leaf and stigma croci with a weight ratio of 1:

0.8-1.5;

0.018-0.030, a step of preparing ginseng extract, a step of preparing ginkgo leaf extract, a step of preparing stigma croci extract, a step of mixing the above ginseng extract, the ginkgo leaf extract and the stigma croci extract.

2. The method according to claim 1, wherein in the step of the preparing ginseng extract, the main component obtained is total ginsenosides;

in the step of preparing Ginkgo leaf extract, the main component obtained is Ginkgo leaf total flavonoids and total lactones;

in the step of preparing stigma croci extract, the main component obtained is stigma croci total glycosides.

3. The method according to claim 2, wherein the steps for preparing the ginseng extract are:

the ginseng is grinded into powders, then subjected to reflux extraction with ethanol for 2 times, filtered, the filtrate obtained is decompressed to recover the solvent until the relative density is 1.12-1.14 at 70°

C., water with the amount that equals to 2-6 times of that of crude drug is added for stirring homogeneously, then it is cooled for precipitation, the supernatant is loaded onto a macroporous adsorptive resin, the resin that carries drug is washed with distilled water first, then is eluted with ethanol, the ethanol eluent is collected and concentrated until dry to obtain the ginseng extract;

the steps for preparing the Ginkgo leaf extract are;

warm ethanol is added to Ginkgo leaf coarse powder for immersion, filtered, and the filter residue is immersed with warm ethanol, filtered again, the filtrates obtained are combined, and concentrated under reduced pressure until the relative density is 1.12-1.14 at 70°

C., water with the amount that equals to 2-6 times of that of crude drug is added, stirred homogeneously, then cooled for precipitation, filtered, the filtrate is loaded onto a macroporous adsorptive resin, the resin that carries drug is washed with distilled water first, then is eluted with ethanol, the ethanol eluent is collected and concentrated until the relative density is 1.02-1.04 at 70°

C., it is extracted by ethyl acetate;

n-butyl alcohol, the extracts are combined and are decompressed to recover solvent to obtain Ginkgo leaf extract; and

the steps for preparing the stigma croci extract are;

cold ethanol is added into stigma croci crude drug for immersion, then filtered, cold ethanol is added to the residues for immersion again, then filtered, the filtrates obtained are combined, concentrated under reduced pressure until the relative density is 1.12-1.14 at 70°

C., water is added, then it is loaded onto a macroporous adsorptive resin, the resin that carries drug is washed with distilled water first, then is eluted with ethanol, the ethanol eluent is concentrated until the relative density is 1.02-1.04 at 70°

C., it is concentrated until dry to obtain stigma croci extract.

4. The method according to claim 1, wherein in the step of weighing ginseng, ginkgo leaf and stigma croci, the weight ratio of ginseng, ginkgo leaf and stigma croci is 1:

1;

0.018-0.030.

5. The method according to claim 1, wherein in the step of weighing ginseng, ginkgo leaf and stigma croci, the weight ratio of ginseng, ginkgo leaf and stigma croci is 1:

0.9-1.2;

0.020-0.025.

6. The method according to claim 1, wherein in the step of weighing ginseng, ginkgo leaf and stigma croci, the weight ratio of ginseng, ginkgo leaf and stigma croci is 1:

1;

0.020-0.025.

7. The method according to claim 1, wherein in the step of weighing ginseng, ginkgo leaf and stigma croci, the weight ratio of ginseng, ginkgo leaf and stigma croci is 1:

1;

0.022.

8. A Chinese medicinal formulation for treating cardiovascular and cerebrovascular diseases and/or dementia which is composed of the Chinese medicinal composition prepared according to the method of claim 1 and at least one pharmaceutically acceptable excipient.

9. A method for treating cardiovascular and cerebrovascular diseases and/or dementia in a subject, comprising administering the Chinese medicinal composition prepared according to the method of claim 1 to a subject in need thereof.

10. The method according to claim 9, wherein the cardiovascular and cerebrovascular disease is selected from the group consisting of ischemic cerebrovascular disease, coronary heart disease, angina, and combinations thereof.

11. The method according to claim 9, wherein the dementia is senile dementia.

12. The method according to claim 11, wherein the senile dementia is vascular dementia.