USE OF A SUGAR TOLERANT BETA-GLUCOSIDASE
First Claim
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1. ) The method of providing a polypeptide having sugar-tolerant beta-glucosidase activity for hydrolyzing a lignocellulosic substrate to yield glucose and/or other sugars,selecting said polypeptide from the group consisting of:
- a) a polypeptide comprising an amino acid sequence at least 50% identical to the amino acid sequence of SEQ ID NO;
2,b) a polypeptide which is encoded by a polynucleotide at least 50% identical to SEQ ID NO;
1,c) a polypeptide which is encoded by a polynucleotide which hybridizes under at least medium stringency conditions with the polynucleotide of SEQ ID NO;
1, or a complementary strand thereof, andd) a fragment of a), b), or c) having beta-glucosidase activity to perform the hydrolysis.
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Abstract
A method of providing a polypeptide having sugar-tolerant beta-glucosidase activity for hydrolysis of a lignocellulosic substrate to yield glucose and/or other sugars. Also provided is a host cell comprising and/or secreting said polypeptide and a kit-of-parts comprising said polypeptide and one or more other cellulases for hydrolyzing a lignocellulosic substrate in a sugar-tolerant manner.
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20 Claims
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1. ) The method of providing a polypeptide having sugar-tolerant beta-glucosidase activity for hydrolyzing a lignocellulosic substrate to yield glucose and/or other sugars,
selecting said polypeptide from the group consisting of: -
a) a polypeptide comprising an amino acid sequence at least 50% identical to the amino acid sequence of SEQ ID NO;
2,b) a polypeptide which is encoded by a polynucleotide at least 50% identical to SEQ ID NO;
1,c) a polypeptide which is encoded by a polynucleotide which hybridizes under at least medium stringency conditions with the polynucleotide of SEQ ID NO;
1, or a complementary strand thereof, andd) a fragment of a), b), or c) having beta-glucosidase activity to perform the hydrolysis. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification