ASPARTIC PROTEASES
First Claim
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1. A recombinant construct comprising a regulatory sequence functional in a production host operably linked to a nucleotide sequence encoding an aspartic protease selected from the group consisting of(a) an aspartic protease having at least 75% sequence identity to SEQ ID NO:
- 2 or SEQ ID NO;
7;
(b) an aspartic protease having at least 78% sequence identity to SEQ ID NO;
6, or SEQ ID NO;
9; and
(c) an aspartic protease having at least 95% sequence identity to SEQ ID NO;
4 or SEQ ID NO;
8.
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Abstract
Novel aspartic proteases, compositions containing these proteases and uses thereof are disclosed.
1 Citation
18 Claims
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1. A recombinant construct comprising a regulatory sequence functional in a production host operably linked to a nucleotide sequence encoding an aspartic protease selected from the group consisting of
(a) an aspartic protease having at least 75% sequence identity to SEQ ID NO: - 2 or SEQ ID NO;
7;(b) an aspartic protease having at least 78% sequence identity to SEQ ID NO;
6, or SEQ ID NO;
9; and(c) an aspartic protease having at least 95% sequence identity to SEQ ID NO;
4 or SEQ ID NO;
8. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- 2 or SEQ ID NO;
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10. Use of an aspartic protease in feed, feedstuff, a feed additive composition or premix wherein the aspartic protease is selected from the group consisting of
(a) an aspartic protease having at least 75% sequence identity to SEQ ID NO: - 2 or SEQ ID NO;
7;(b) an aspartic protease having at least 78% sequence identity to SEQ ID NO;
6, or SEQ ID NO;
9; and(c) an aspartic protease having at least 95% sequence identity to SEQ ID NO;
4 or SEQ ID NO;
8,wherein the aspartic protease may be used (i) alone or (ii) in combination with a direct fed microbial comprising at least one bacterial strain or (iii) with at least one other enzyme or (iv) in combination with a direct fed microbial comprising at least one bacterial strain and at least one other enzyme.
- 2 or SEQ ID NO;
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11. Animal feed comprising an aspartic protease is selected from the group consisting of:
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(a) an aspartic protease having at least 75% sequence identity to SEQ ID NO;
2 or SEQ ID NO;
7;(b) an aspartic protease having at least 78% sequence identity to SEQ ID NO;
6, or SEQ ID NO;
9; and(c) an aspartic protease having at least 95% sequence identity to SEQ ID NO;
4 or SEQ ID NO;
8,wherein the aspartic protease is present in an amount from 1-20 g/ton feed and further wherein the aspartic protease may be used (i) alone or (ii) in combination with a direct fed microbial comprising at least one bacterial strain or (iii) with at least one other enzyme or (iv) in combination with a direct fed microbial comprising at least one bacterial strain and at least one other enzyme.
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12. An isolated polypeptide having protease activity, said polypeptide comprising an amino acid sequence protease selected from the group consisting of:
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(a) an aspartic protease having at least 75% sequence identity to SEQ ID NO;
2 or SEQ ID NO;
7;(b) an aspartic protease having at least 78% sequence identity to SEQ ID NO;
6, or SEQ ID NO;
9; and(c) an aspartic protease having at least 95% sequence identity to SEQ ID NO;
4 or SEQ ID NO;
8. - View Dependent Claims (14, 15, 16, 17, 18)
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13. A polynucleotide sequence encoding the polypeptide of claim 13.
Specification