Methods of Generating Libraries of Nucleic Acid Sequences for Detection via Fluorescent in Situ Sequ
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Abstract
The present disclosure provides a number of targeted nucleic acid FISSEQ library construction methods. Targeted FISSEQ can exhibit several benefits, such as enhanced sensitivity and/or shorter assay time in the detection, identification, quantification, and/or determining the nucleotide sequence of the target species, relative to “random” or “whole-omic” detection via FISSEQ.
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Citations
122 Claims
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1-102. -102. (canceled)
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103. A method for in situ nucleic acid sequence detection or identification of one or more target nucleic acid molecules of a cell, comprising:
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(a) providing said cell comprising a reaction mixture comprising said one or more target nucleic acid molecules and a plurality of probes, wherein said plurality of probes comprises a plurality of target-specific sequences, a plurality of adaptor sequences and a plurality of barcode sequences, wherein a probe of said plurality of probes comprises;
(i) a target-specific sequence of said plurality of target-specific sequences that is complementary to a target sequence of a target nucleic acid molecule of said one or more target nucleic acid molecules;
(ii) an adaptor sequence of said plurality of adaptor sequences coupled to said target-specific sequence, wherein said adaptor sequence is for conducting an amplification reaction on said probe when said target-specific sequence is hybridized to said target sequence; and
(iii) a barcode sequence of said plurality of barcode sequences coupled to said adaptor sequence, wherein said barcode sequence is configured to allow detection or identification of said target sequence or at least a portion of said target nucleic acid molecule, and wherein said plurality of barcode sequences are different across said plurality of probes;(b) within said cell, subjecting said reaction mixture to conditions sufficient to permit said target-specific sequence to hybridize to said target sequence; and (c) using said barcode sequence to detect or identify said target sequence or said at least said portion of said target nucleic acid molecule. - View Dependent Claims (104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120)
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121. A method for enhancing a hybridization reaction in a cell or cellular matrix, comprising:
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(a) providing said cell or cellular matrix and a reaction mixture, comprising (i) a target nucleic acid molecule, (ii) a probe having sequence complementarity with a target sequence of said target nucleic acid molecule, and (iii) a hybridization reaction enhancing agent comprising a polymer backbone, wherein said hybridization reaction enhancing agent enhances a rate of a hybridization reaction between said target nucleic acid molecule and said probe having sequence complementarity with said target sequence of said target molecule; (b) subjecting said reaction mixture to conditions sufficient to conduct said hybridization reaction between said target nucleic acid molecule and said probe having sequence complementarity with said target sequence of said target nucleic acid molecule, wherein during said hybridization reaction, said hybridization reaction enhancing agent facilitates said hybridization reaction between said target nucleic acid molecule and said probe having sequence complementarity with said target sequence of said target molecule; and (c) removing said hybridization reaction enhancing agent from said cell or cellular matrix. - View Dependent Claims (122)
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Specification