PLURIPOTENT STEM CELLS OBTAINED BY NON-VIRAL REPROGRAMMING

• US 20190330654A1
• Filed: 12/04/2018
• Published: 10/31/2019
• Est. Priority Date: 10/24/2008
• Status: Abandoned Application

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Thomson, James, Yu, Junying
• Application Number:
  US16/209,722
• Publication Number:
  US 20190330654A1
• Time in Patent Office: Days
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2501/602 Sox-2
• CPC Class Codes: C12N 2501/603 Oct-3/4
• CPC Class Codes: C12N 2501/604 Klf-4
• CPC Class Codes: C12N 2501/605 Nanog
• CPC Class Codes: C12N 2501/606 c-Myc
• CPC Class Codes: C12N 2501/608 Lin28
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0696 Artificially induced pluripotent stem cells, e.g. iPS

Abstract

Methods for reprogramming primate somatic cells to pluripotency using an episomal vector that does not encode an infectious virus are disclosed. Pluripotent cells produced in the methods are also disclosed.

Claims

1-20. -20. (canceled)

21. An in vitro population of primate pluripotent cells produced according to the method of:

a) introducing a plurality of plasmids into primate somatic cells in vitro; and

b) culturing the cells obtained in step a) under conditions such that a population of pluripotent cells are obtained, wherein the plurality of plasmids are selected from the group consisting of;

(i) pEP4-E-O2S-E-T2K, pEP4-E-O2S-E-N2K and pCEP4-M2L;

(ii) pEP4-E-O2S-C-K2M-E-N2L and pEP4-E-O2S-E-T2K; and

(iii) pEP4-E-O2S-E-N2L, pEP4-E-O2S-E-T2K and pEP4-E-O2S-E-M2K, wherein-pEP4 and pCEP4 are plasmids, E is an EF1α

promoter;

O is an OCT4 coding region, S is a SOX2 coding region, T is an SV40T antigen, coding region, N is a NANOG coding region, K is a KLF4 coding region, M is a c-Myc coding region, C is a CMV promoter and L is a LIN28 coding region; and

wherein pluripotent cells in the population comprise at least one plasmid of the plurality of plasmids.

22. The population of claim 21, wherein the primate somatic cells are human somatic cells.

23. The population of claim 21, wherein OCT4 and SOX2 are human.

24. The population of claim 21, wherein LIN28, NANOG, c-Myc, and KLF4 are human.