PRODUCTION OF ITACONIC ACID AND RELATED MOLECULES FROM AROMATIC COMPOUNDS
First Claim
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1. A genetically-modified bacterium from the genus Pseudomonas comprising an exogenous nucleic acid encoding an enzyme that uses cis-aconitate as a substrate.
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Abstract
This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that produces itaconate or trans-aconitate. The disclosure further provides methods for producing itaconate or trans-aconitate using a genetically-modified bacterium from the genus Pseudomonas.
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36 Claims
- 1. A genetically-modified bacterium from the genus Pseudomonas comprising an exogenous nucleic acid encoding an enzyme that uses cis-aconitate as a substrate.
- 19. A method for converting an organic compound to itaconic acid or trans-aconitate, the method comprising inoculating an aqueous solution containing said organic compound with a genetically-modified bacterium from the genus Pseudomonas, wherein the bacterium comprises an exogenous nucleic acid encoding an enzyme that uses cis-aconitate as a substrate.
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