Self-Assembled Nanoplatelet-Enzyme Bioconjugates Providing for Increased Biocatalytic Efficiency
First Claim
Patent Images
1. An enzymatic cascade cluster comprising:
- a plurality of nanoplatelets associated together as a cluster, wherein each nanoplatelet is bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth; and
wherein the enzymatic cascade comprises at least two different enzymes; and
wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter.
1 Assignment
0 Petitions
Accused Products
Abstract
A nanoplatelet serves as a substrate for immobilizing enzymes involved in consecutive reactions as a cascade. This results in a significant increase in the rate of catalysis as well as final product yield compared to non-immobilized enzymes or enzymes immobilized to quantum dots.
0 Citations
13 Claims
-
1. An enzymatic cascade cluster comprising:
-
a plurality of nanoplatelets associated together as a cluster, wherein each nanoplatelet is bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth; and wherein the enzymatic cascade comprises at least two different enzymes; and wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter. - View Dependent Claims (2, 3)
-
-
4. An enzymatic cascade comprising:
-
a nanoplatelet bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth; wherein the enzymatic cascade comprises at least two different enzymes. - View Dependent Claims (5, 6)
-
-
7. An enzymatic cascade cluster comprising:
-
a plurality of nanoplatelets associated together as a cluster, wherein each nanoplatelet is bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth; wherein the enzymatic cascade consists of the enzymes glucokinase, phosphoglucose isomerase, phosphofructokinase, aldolase, triose phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, and phophoglycerate kinase, each enzyme incorporating a polyhistidine sequence associating the enzyme to a nanoplatelet; and wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter.
-
-
8. A method of conducting a cascade reaction, comprising:
-
providing a cascade cluster comprising a plurality of nanoplatelets associated together as a cluster, wherein each nanoplatelet is bound to a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth, wherein the enzymatic cascade comprises at least two different enzymes, and wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter; contacting the cascade cluster with a substrate of the first enzyme; and allowing a reaction to proceed so that each of the plurality of enzymes acts in succession to produce an end product, wherein the reaction is performed while minimizing stirring or mixing. - View Dependent Claims (9, 10)
-
-
11. A method of preparing an enzymatic cascade cluster, the method comprising:
contacting a plurality of nanoplatelets with a plurality of enzymes configured as an enzymatic cascade wherein the product of a first enzyme is the substrate of a second enzyme and so forth, wherein the enzymatic cascade comprises at least two different enzymes;
thereby forming an enzymatic cascade cluster wherein the nanoplatelets in the cluster are closely associated with one another such that, on average, each nanoplatelet is separated from the nearest neighboring nanoplatelet by a distance of no more than about one nanoplatelet diameter.- View Dependent Claims (12, 13)
Specification