ACIDOPHILIC FUSARIUM OXYSPORUM STRAINS, METHODS OF THEIR PRODUCTION AND METHODS OF THEIR USE

• US 20190330667A1
• Filed: 05/22/2019
• Published: 10/31/2019
• Est. Priority Date: 07/03/2014
• Status: Abandoned Application

• Current Assignee: Fynder Group Incorporated
• Original Assignee: Sustainable Bioproducts, Inc.
• Inventors: KOZUBAL, Mark A., MACUR, Richard E., INSKEEP, William P.
• Application Number:
  US16/419,986
• Publication Number:
  US 20190330667A1
• Time in Patent Office: Days
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12N 1/22 Processes using, or culture media containing, cellulose or hydrolysates thereof
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 3/00 Preparation of elements or inorganic compounds except carbon dioxide recovery of carbon dioxides as by-products C12F3/02
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: C12P 7/6458 by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
• CPC Class Codes: C12P 7/6463 obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/649 Biodiesel, i.e. fatty acid alkyl esters
• CPC Class Codes: C12R 2001/77 Fusarium
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present invention provides isolated acidophilic Fusarium oxysporum strains, such as MK7, and their progeny, compositions comprising such strains and their progeny, methods of producing such strains and their progeny, and methods of using such strains and their progeny.

Claims

1-20. -20. (canceled)

21. A method of producing one or more energy-rich metabolites, said method comprising the steps of:

(a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698), and/or its progeny, with a wheat straw feedstock material that supports the growth of the Fusarium oxysporum strain MK7 and/or its progeny;

(b) adding manganese to the mixture to a final concentration of 5-25 mM;

(c) incubating said mixture at pH 0.5-8.0 for a period of time under anaerobic conditions to produce fungal biomass;

(d) producing from the mixture and/or fungal biomass one or more energy-rich metabolites following such contact, wherein production of the one or more energy-rich metabolites is improved compared to a control lacking manganese; and

(e) optionally, isolating said one or more energy-rich metabolites from the mixture.

22. The method of claim 21, wherein the pH is less than 3.0.

23. A method of producing one or more energy-rich metabolites, said method comprising the steps of:

(a) making a mixture by contacting an isolated Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698), and/or its progeny, with a wheat straw feedstock material that supports the growth of the Fusarium oxysporum strain MK7 and/or its progeny;

(b) adding manganese peroxide isolated from Fusarium oxysporum strain MK7 (ATCC Accession Deposit No. PTA-10698) to the mixture;

(c) incubating said mixture at a pH of less than 3.0 for a period of time under anaerobic conditions to produce fungal biomass;

(d) producing from the mixture and/or fungal biomass one or more energy-rich metabolites following such contact, wherein production of the one or more energy-rich metabolites is improved compared to a control lacking manganese peroxidase isolated from Fusarium oxysporum strain MK7; and

(e) optionally, isolating said one or more energy-rich metabolites from the mixture.

24. A method of culturing a Fusarium strain, comprising:

(a) culturing a biologically pure culture of Fusarium MK7 strain, deposited as ATCC Accession Deposit No. PTA-10698, in a liquid culture media comprising a carbon source and having pH of less than 5.0 to produce Fusarium MK7 strain in a form comprising hyphae of Fusarium MK7 strain; and

(b) harvesting the Fusarium MK7 strain.

25. The method of claim 24, wherein the liquid culture media further comprises an amino acid donor.