BIOSENSOR AND BIOLOGICAL DETECTION METHOD

• US 20190331631A1
• Filed: 04/30/2019
• Published: 10/31/2019
• Est. Priority Date: 04/30/2018
• Status: Active Grant

• Current Assignee: Industrial Technology Research Institute
• Original Assignee: Industrial Technology Research Institute
• Inventors: Hsieh, Hui-Chu, Hsieh, Wen-Pin, Chou, Nien-Jen, Shen, Hui-Ju, Peng, Hui-Ling, Lin, Jing-Tsyr
• Granted Patent: US 11,131,644 B2
• Time in Patent Office: Days
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3276 being a hybridisation with immobilised receptors using a FET type sensor G01N27/4145; concerning the hybridisation C12Q1/68
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

A biosensor and a biological detection method are provided. The biosensor includes a substrate, a plurality of first and second electrodes in a strip shape, a material layer and a capture antibody. The substrate has a sampling region and a measuring region connected to the sampling region. The first and second electrodes are disposed on the substrate in the measuring region, and the first and second electrodes are arranged alternately with each other, wherein the first electrodes are connected in parallel with each other, and the second electrodes are connected in parallel with each other. The material layer is disposed on the substrate in the measuring region and covers the first and second electrodes, wherein the material layer has a channel exposing a portion of each of the first electrodes and a portion of each of the second electrodes, the channel is connected to the sampling region, and an extending direction of the channel is interlaced with an extending direction of the first electrodes and the second electrodes. The capture antibody is disposed on the first and second electrodes exposed by the channel.

Claims

1. An biosensor, comprising:

a substrate, having a sampling region and a measuring region connected to the sampling region;

a plurality of first electrodes in a strip shape and a plurality of second electrodes in a strip shape, disposed on the substrate in the measuring region, wherein the plurality of first electrodes and the plurality of second electrodes are arranged alternately with each other, the plurality of first electrodes are connected in parallel with each other, and the plurality of second electrodes are connected in parallel with each other;

a material layer, disposed on the substrate in the measuring region and covering the plurality of first electrodes and the plurality of second electrodes, wherein the material layer comprises a channel exposed a portion of each of the plurality of first electrodes and a portion of each of the plurality of second electrodes, the channel is connected to the sampling region, and an extending direction of the channel is interlaced with an extending direction of the plurality of first electrodes and the plurality of second electrodes; and

a capture antibody, disposed on the plurality of first electrodes exposed by the channel and the plurality of second electrodes exposed by the channel.

2. The biosensor according to claim 1, further comprising:

a cover, disposed on the material layer and covering the measuring region; and

a first hydrophilic layer, disposed on a surface of the cover facing the channel.

3. The biosensor according to claim 1, wherein the substrate comprises a collecting region, the collecting region is connected to the channel, and the measuring region is disposed between the collecting region and the sampling region.

4. The biosensor according to claim 3, wherein the collecting region comprises a groove disposed in the substrate.

5. The biosensor according to claim 4, further comprising:

an absorbing material, disposed on a bottom and side walls of the groove.

6. The biosensor according to claim 3, wherein the channel is S-shaped in the measuring region adjacent to the collecting region.

7. The biosensor according to claim 5, further comprising:

a second hydrophilic layer, disposed on the absorbing material.

8. The biosensor according to claim 1, wherein a sum of lengths of overlapping portions of the channel with the plurality of first electrodes and the plurality of second electrodes in the extending direction of the channel is greater than or equal to 0.8 cm.

9. The biosensor according to claim 1, wherein a width of the channel is between 1.5 mm to 3 mm.

10. The biosensor according to claim 1, further comprising:

a third electrode, disposed on the substrate in the sampling region near a boundary of the measuring region.

11. The biosensor according to claim 1, wherein the sampling region comprises a groove disposed in the substrate.

12. A biological detection method, comprising:

providing a biosensor according to claim 1;

providing a sample having an antigen to the sampling region and filling the channel with the sample such that the antigen binds to the capture antibody;

providing a voltage difference between the plurality of first electrodes and the plurality of second electrodes to cause an electrochemical reaction between the antigen and the capture antibody; and

collecting a current generated by the electrochemical reaction to obtain a concentration of the antigen corresponding to the current.

13. A biological detection method, comprising:

providing a biosensor according to claim 1;

providing a sample having an antigen to the sampling region and filling the channel with the sample such that the antigen binds to the capture antibody;

providing a detection antibody with an enzyme to the sampling region and filling the channel with the detection antibody such that the antigen binds to the detection antibody;

providing a cleaning solution into the channel;

providing an enzyme substrate into the channel to cause the enzyme substrate to react with the enzyme;

providing a voltage difference between the plurality of first electrodes and the plurality of second electrodes to cause an electrochemical reaction of an enzyme product produced by the enzyme reaction; and

collecting a current generated by the electrochemical reaction to obtain a concentration of the antigen corresponding to the current.