DETECTION DEVICE FOR ION COUNT IN TUMOR-RELATED MOLECULES AND USAGE METHOD THEREOF

• US 20190331641A1
• Filed: 06/30/2019
• Published: 10/31/2019
• Est. Priority Date: 11/02/2017
• Status: Active Grant

• Current Assignee: Jian Wang
• Original Assignee: Jian Wang
• Inventors: WANG, Jian
• Granted Patent: US 10,859,530 B2
• Time in Patent Office: Days
• CPC Class Codes: G01N 27/64 using wave or particle radiation to ionise a gas, e.g. in an ionisation chamber
• CPC Class Codes: G01N 27/66 and measuring current or voltage
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735

Abstract

Disclosed is a detection device for an ion count in tumor-related molecules and a usage method thereof. The detection device comprises an air sample chamber (1), a first solenoid valve (2), an ionization chamber (3), a filament (4), a second solenoid valve (5), a vacuum generator (6), a high-voltage accelerating electrode (7), an ion collecting electrode (8) and an ion counter (9). The air sample chamber (1) is connected to the ionization chamber (3) through the first solenoid valve (2). The filament (4) is arranged at a left end of the ionization chamber (3). A lower end of the ionization chamber (3) is connected to the vacuum generator (6) through the second solenoid valve (5). The high-voltage accelerating electrode (7) and the ion collecting electrode (8) are successively arranged at a right end of the ionization chamber (3). The ion collecting electrode (8) is connected to the ion counter (9).

Claims

1. A detection device for an ion count in tumor-related molecules, comprising an air sample chamber (1), a first solenoid valve (2), an ionization chamber (3), a filament (4), a second solenoid valve (5), a vacuum generator (6), a high-voltage accelerating electrode (7), an ion collecting electrode (8) and an ion counter (9), wherein the air sample chamber (1) is connected to the ionization chamber (3) through the first solenoid valve (2);

the filament (4) is arranged at a left end of the ionization chamber;

a lower end of the ionization chamber (3) is connected to the vacuum generator (6) through the second solenoid valve (5);

the high-voltage accelerating electrode (7) and the ion collecting electrode (8) are successively arranged at a right end of the ionization chamber (3);

the ion collecting electrode (8) is connected to the ion counter (9);

the air sample chamber (1) is configured to collect the air exhaled by a subject;

the first solenoid valve (2) is communicated with the air sample chamber (1) and the ionization chamber (3), and configured to control the flow of the air from the air sample chamber (1) to the ionization chamber (3); and

the filament (4) is connected to a negative pole of a high-voltage electric field, to serve as a cathode.

2. The detection device for an ion count in tumor-related molecules according to claim 1, wherein the high-voltage accelerating electrode (7) is of a net structure.

3. The detection device for an ion count in tumor-related molecules according to claim 1, wherein the ion collecting electrode (8) is a graphene electrode.

4. A method for using the detection device for an ion count in tumor-related molecules according claim 1, comprising steps:

1) vacuumizing;

during which, the second solenoid valve (5) is turned on, and the ionization chamber (3) is vacuumized by the vacuum generator (6) until the degree of vacuum inside the ionization chamber (3) reaches a set standard;

2) starting pre-heating of the filament (4) to generate an electron source;

during which, the ionization high voltage is turned on, and the filament (4) is connected to a negative pole of a high-voltage electric field so that the filament (4), after being powered on and heated, emits electrons to the ionization chamber (3) to form a stream of electrons;

3) accelerating the electrons;

during which, a positive voltage is applied onto the high-voltage accelerating electrode (7), the positive voltage, serving as an accelerating electrode high voltage, draws and accelerates the electrodes emitted by the filament (4), and the accelerated electrons are repelled by the ion collecting electrode (8) after passing through the high-voltage accelerating electrode (7) since a voltage at the ion collecting electrode (8) is negative with respect to the high-voltage accelerating electrode (7), and then return to the high-voltage accelerating electrode (7) in an accelerated speed;

4) ionizing the air;

during which, the probability of collision of the electrons with air molecules is increased in the return motion of the electrons so that more air molecules are ionized to become positive ions and secondary electrons, and the electronic interaction between the high-speed electrons and the tumor-related molecules in atomic orbits is realized by regulating the high-voltage electric field since the orbital electrons outside the nucleus have their own characteristic energy levels; and

the electrons are forced out of the orbits to obtain ions having an energy spectrum representative of characteristics of the orbits; and

5) ion counting;

during which, the positive ions are drawn by the ion collecting electrode (8) with a lowest potential;

an induced voltage is formed on the ion collecting electrode (8), and the induced voltage is related to corresponding ions; and

the induced voltage is passed to the ion counter (9) which makes a corresponding determination, so far one measurement is completed.

5. The method for using the detection device for an ion count in tumor-related molecules according to claim 4, wherein, in the step 4), when an air sample to be tested is found in the air sample chamber (1), the CPU controls to turn on the first solenoid valve (2), and turn off the first solenoid valve (2) when the air is fed to the ionization chamber (3);

and in the step

     5), when the ionization high voltage is turned on, the ion counter (9) starts counting, and at the end of counting, an ion count curve corresponding to related molecules is shown in a display; and

the vacuum generator (6) is configured to maintain the measurement condition in the ionization chamber.

6. The method for using the detection device for an ion count in tumor-related molecules according to claim 5, further comprising sorted detection of tumor-related molecules, comprising:

regulating a voltage value of the accelerating electrode high voltage, ionizing tumor molecules by different voltages, and analyzing the result of ionization by the ion counter (9).