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MOLECULAR BARCODING ON OPPOSITE TRANSCRIPT ENDS

  • US 20190338278A1
  • Filed: 05/01/2019
  • Published: 11/07/2019
  • Est. Priority Date: 05/03/2018
  • Status: Active Grant
First Claim
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1. A method for analyzing a nucleic acid target in a sample, comprising:

  • barcoding copies of the nucleic acid target in a sample using a plurality of oligonucleotide barcodes to generate a plurality of barcoded nucleic acid molecules each comprising a sequence of the nucleic acid target, a molecular label, and a target-binding region, and wherein at least 10 of the plurality of oligonucleotide barcodes comprise different molecular label sequences;

    attaching an oligonucleotide comprising a complement of the target binding region to the plurality of barcoded nucleic acid molecules to generate a plurality of barcoded nucleic acid molecules each comprising the target-binding region and the complement of the target-binding region;

    hybridizing the target-binding region and the complement of the target-binding region within each of the plurality of barcoded nucleic acid molecules to form a stem loop; and

    extending 3′

    -ends of the plurality of barcoded nucleic acid molecules to extend the stem loop to generate a plurality of extended barcoded nucleic acid molecules each comprising the molecular label and a complement of the molecular label.

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