ANALYSIS DEVICE, ANALYSIS METHOD, AND PROGRAM

• US 20200043159A1
• Filed: 10/03/2016
• Published: 02/06/2020
• Est. Priority Date: 10/03/2016
• Status: Abandoned Application

• Current Assignee: Nikon Corporation
• Original Assignee: Nikon Corporation
• Inventors: WATANABE, Hirotada, MAIYA, Nobuhiko, TAKEI, Shunsuke, SAIGO, Takuro, MASUTANI, Mamiko, FURUTA, Shinichi, YAMASHITA, Masafumi, YAMASAKI, Shoko, OTSUBO, Yosuke
• Application Number:
  US16/338,618
• Publication Number:
  US 20200043159A1
• Time in Patent Office: Days
• CPC Class Codes: C12M 1/34 Measuring or testing with condition measuring or sensing means, e.g. colony counters
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 21/17 Systems in which incident light is modified in accordance with the properties of the material investigated where the material investigated is optically excited causing a change in wavelength of the incident light G01N21/63
• CPC Class Codes: G01N 33/483 Physical analysis of biological material
• CPC Class Codes: G06T 2207/10056 Microscopic image
• CPC Class Codes: G06T 2207/30024 Cell structures in vitro; Tissue sections in vitro
• CPC Class Codes: G06T 7/0012 Biomedical image inspection
• CPC Class Codes: G06V 20/695 Preprocessing, e.g. image segmentation
• CPC Class Codes: G06V 20/698 Matching; Classification

Abstract

An analysis device includes a living cell feature value extraction unit configured to extract a feature value of a living cell from a captured image of the living cell, a fixed cell feature value extraction unit configured to extract a feature value of a fixed cell from a captured image of a cell that is the fixed cell of the living cell, and a computing unit configured to associate a feature value of the living cell extracted by the living cell feature value extraction unit with a feature value of the fixed cell extracted by the fixed cell feature value extraction unit.

Claims

1. An analysis device configured to analyze a correlation between feature values within a cell in response to a stimulus, the analysis device comprising:

a living cell feature value extraction unit configured to extract a feature value of a living cell from a captured image of the living cell;

a fixed cell feature value extraction unit configured to extract a feature value of a fixed cell from a captured image of a cell that is the fixed cell of the living cell; and

a computing unit configured to associate a feature value of the living cell extracted by the living cell feature value extraction unit with a feature value of the fixed cell extracted by the fixed cell feature value extraction unit.

2. The analysis device according to claim 1, further comprising:

a correlation calculation unit configured to calculate a correlation between feature values within a cell in response to the stimulus from a feature value of the fixed cell and a feature value of the living cell.

3. The analysis device according to claim 2, wherein a correlation between feature values within a cell in response to the stimulus is calculated from a plurality of captured images of cells fixed with different elapsed times in response to the stimulus.

4. The analysis device according to claim 3, wherein a feature value of a living cell is extracted from the living cell corresponding to a cell fixed with the different elapsed time, and a correlation between feature values within a cell in response to the stimulus is calculated.

5. The analysis device according to claim 2, further comprising:

a correlation extraction unit configured to be indicated by the correlation based on biological information of the feature value with respect to a correlation between feature values within a cell calculated by the correlation calculation unit.

6. The analysis device according to claim 1, wherein the feature value of the living cell is a dynamic feature value.

7. The analysis device according to claim 1, further comprising:

a microscope configured to image capture the cell.

8. The analysis device according to claim 1, wherein the living cell feature value extraction unit specifies a feature value of the living cell from a captured image of the living cell after the stimulus is applied.

9. The analysis device according to claim 1, wherein the living cell feature value extraction unit specifies a feature value of the living cell from a captured image of the living cell before the stimulus is applied.

10. The analysis device according to claim 1, wherein a cell corresponding to a living cell extracted by the living cell feature value extraction unit is extracted from a captured image of a cell that is a fixed cell of the living cell.

11. An analysis method performed by an analysis device configured to analyze a correlation between feature values within a cell in response to a stimulus, the analysis method comprising:

extracting a feature value of a living cell from a captured image of the living cell;

extracting a feature value of a fixed cell from a captured image of a cell that is the fixed cell of the living cell; and

computing to associate a feature value of the living cell extracted in the extracting of the feature value of the living cell with a feature value of the fixed cell extracted in the extracting of the feature value of the fixed cell.

12. A program configured to cause a computer of an analysis device to perform:

extracting a feature value of a living cell from a captured image of the living cell;

extracting a feature value of a fixed cell from a captured image of a cell that is the fixed cell of the living cell; and

computing to associate a feature value of the living cell extracted in the extracting of the feature value of the living cell with a feature value of the fixed cell extracted in the extracting of the feature value of the fixed cell.