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RNA-Guided Human Genome Engineering

  • US 20200048656A1
  • Filed: 06/13/2019
  • Published: 02/13/2020
  • Est. Priority Date: 12/17/2012
  • Status: Active Grant
First Claim
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1. A method of altering a eukaryotic cell comprisingtransfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell,transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner,wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

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