NOVEL POLYMERS AND DNA COPOLYMER COATINGS

• US 20200131285A1
• Filed: 01/10/2020
• Published: 04/30/2020
• Est. Priority Date: 10/31/2014
• Status: Active Grant

• Current Assignee: Illumina Cambridge Ltd. (California) (Illumina Incorporated)
• Original Assignee: Illumina Cambridge Ltd. (California) (Illumina Incorporated)
• Inventors: Brown, Andrew A., George, Wayne N., Richez, Alexandre, Dingwall, Anne-Cecile, von Hatten, Xavier
• Granted Patent: US 11,447,582 B2
• Time in Patent Office: Days
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C08F 20/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/22 Esters containing halogen
• CPC Class Codes: C08F 220/282 and containing two or more oxygen atoms
• CPC Class Codes: C08F 220/325 containing glycidyl radical, e.g. glycidyl (meth)acrylate
• CPC Class Codes: C08F 220/346 and further oxygen
• CPC Class Codes: C08F 220/56 Acrylamide; Methacrylamide
• CPC Class Codes: C08F 220/60 containing nitrogen in addition to the carbonamido nitrogen
• CPC Class Codes: C08F 220/603 and containing oxygen in addition to the carbonamido oxygen and nitrogen
• CPC Class Codes: C08F 222/385 Monomers containing two or more (meth)acrylamide groups, e.g. N,N'-methylenebisacrylamide
• CPC Class Codes: C08F 2438/03 Use of a di- or tri-thiocarbonylthio compound, e.g. di- or tri-thioester, di- or tri-thiocarbamate, or a xanthate as chain transfer agent, e.g . Reversible Addition Fragmentation chain Transfer [RAFT] or Macromolecular Design via Interchange of Xanthates [MADIX]
• CPC Class Codes: C08F 285/00 Macromolecular compounds obtained by polymerising monomers on to preformed graft polymers
• CPC Class Codes: C08F 293/005 using free radical "living" or "controlled" polymerisation, e.g. using a complexing agent
• CPC Class Codes: C08F 8/32 by reaction with amines
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2565/401 Signal amplification by chemical polymerisation
• CPC Class Codes: C40B 50/18 using a particular method of attachment to the solid support

Abstract

Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.

Claims

1. A polymer for surface functionalization, comprising a recurring unit of Formula (V):

2. The polymer of claim 1, wherein R^1d is hydrogen or alkyl.

3. The polymer of claim 1, wherein each R^2d and R^3d is hydrogen.

4. The polymer of claim 1, wherein R^B is azido, amino, or Boc-protected amino, or combinations thereof.

5. The polymer of claim 1, wherein L⁴ is a methylene linker.

6. The polymer of claim 1, wherein L⁵ is

7. The polymer of claim 1, wherein the recurring unit of Formula (V) is also represented by Formula (Va) or (Vb), or both:

8. The polymer of claim 1, further comprises a recurring unit of Formula (VIa) or (VIb), or both:

9. The polymer of claim 8, wherein each R^1e and R^1f is hydrogen or alkyl;

and each R^2e and R^2f is hydrogen.

10. A substrate having a first surface comprising a polymer of claim 1 covalently bonded thereto.

11. The substrate of claim 10, further comprising one or more recurring units selected from the group consisting of polyacrylamides, polyacrylates, polyurethanes, polysiloxanes, silicones, polyacroleins, polyphosphazenes, polyisocyanates, poly-ols, and polysaccharides, and combinations thereof.

12. The substrate of claim 10, wherein the polymer is covalently bonded to the first surface of the substrate through reaction with a first plurality of functional groups covalently attached thereto the first surface, wherein the first plurality of functional groups comprise vinyl, acryloyl, alkenyl, cycloalkenyl, heterocycloalkenyl, alkynyl, cycloalkynyl, heterocycloalkynyl, nitrene, aldehyde, hydrazinyl, glycidyl ether, epoxy, carbene, isocyanate or maleimide, or optionally substituted variants or combinations thereof.

13. The substrate of claim 12, wherein the first plurality of functional groups comprise optionally substituted norbornene, glycidyl ether, or epoxy, or combinations thereof

14. The substrate of claim 12, wherein the covalent bonding between the polymer and the first surface of the substrate comprises the structure moiety

15. The substrate of claim 12, wherein the covalent bonding between the polymer and the first surface comprises the structure moiety

16. The substrate of claim 10, further comprising functionalized oligonucleotides covalently bonded to the polymer.

17. The substrate of claim 16, wherein the functionalized oligonucleotides are covalently bonded to the polymer through reaction of one or more functional moieties of the functionalized oligonucleotides with the epoxy groups of the polymer, and wherein said one or more functional moieties of the oligonucleotides comprises optionally substituted amino, hydroxy, thiol, carboxyl, acid anhydride, or combinations thereof.

18. The substrate of claim 16, wherein the covalent bonding between the functionalized oligonucleotide and the polymer comprises the structure moiety

19. A method for immobilizing a polymer to a first surface of a substrate, comprising:

contacting a polymer comprising a recurring unit of Formula (V) with the first surface of the substrate, said first surface comprising a first plurality of functional groups covalently attached thereto; and

reacting the first plurality of functional groups of the first surface with the polymer, thereby covalently bonding the polymer to the first surface of the substrate;

20. The method of claim 19, wherein the first plurality of functional groups of the first surface comprise vinyl, acryloyl, alkenyl, cycloalkenyl, heterocycloalkenyl, alkynyl, cycloalkynyl, heterocycloalkynyl, nitrene, aldehyde, hydrazinyl, glycidyl ether, epoxy, carbene, isocyanate or maleimide, or optionally substituted variants, or combinations thereof.

21. The method of claim 20, wherein the first plurality of functional groups comprise norbornene, glycidyl ether or epoxy, or combinations thereof.

22. The method of claim 21, wherein R^B is azido, amino, or Boc-protected amino, or combinations thereof.