CELL CULTURE VESSEL
First Claim
1. A cell culture vessel comprising:
- a wall comprising inner surfaces defining a cell culture chamber of the vessel;
a substrate of non-porous material positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber, the substrate comprising a plurality of microcavities, each microcavity of the plurality of microcavities comprising a concave surface defining a well and an opening defining a path from the first region into the well, the concave surface of each microcavity comprising at least one aperture comprising a dimension less than or equal to about 15 microns defining a path from the well to the second region; and
a first aperture extending through the wall in fluid communication with the first region.
1 Assignment
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Accused Products
Abstract
A cell culture vessel (100) includes a wall including an inner surface defining a cell culture chamber of the vessel. A substrate (215) of non-porous material is positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber. The substrate includes a plurality of microcavities (220), each microcavity of the plurality of microcavities includes a concave surface defining a well and an opening, the concave surface of each microcavity includes at least one aperture including a dimension less than or equal to about 15 microns defining a path from the well to the second region. A cell culture vessel including a substrate including a plurality of microcavities and a layer of porous material is also provided. Methods of culturing cells in the cell culture vessel are also provided.
3 Citations
34 Claims
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1. A cell culture vessel comprising:
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a wall comprising inner surfaces defining a cell culture chamber of the vessel; a substrate of non-porous material positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber, the substrate comprising a plurality of microcavities, each microcavity of the plurality of microcavities comprising a concave surface defining a well and an opening defining a path from the first region into the well, the concave surface of each microcavity comprising at least one aperture comprising a dimension less than or equal to about 15 microns defining a path from the well to the second region; and a first aperture extending through the wall in fluid communication with the first region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A cell culture vessel comprising:
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a wall comprising an inner surface defining a cell culture chamber of the vessel; a substrate positioned in the cell culture chamber between a first region of the cell culture chamber and a second region of the cell culture chamber, the substrate comprising a plurality of microcavities and a layer of porous material, each microcavity of the plurality of microcavities comprising a concave surface defining a well and an opening defining a path from the first region into the well, wherein a first side of the layer of porous material defines at least a portion of the concave surface of each microcavity of the plurality of microcavities, and wherein a second side of the layer of porous material faces the second region; and a first aperture extending through the wall in fluid communication with the first region. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
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Specification