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Cell Colony Picking System

  • US 20200131465A1
  • Filed: 06/04/2018
  • Published: 04/30/2020
  • Est. Priority Date: 06/02/2017
  • Status: Active Grant
First Claim
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1. A method for selecting one or more monoclonal cell colonies using an apparatus having a digital imaging device, the method comprising:

  • loading a sample plate onto the apparatus, the sample plate including a well having a plurality of cells held in a semi-solid medium;

    capturing, at a first time and using the digital imaging device, a first plurality of vertically spaced-apart images of the well, each image having a different height along a Z axis, such that a volumetric image stack is generated with respect to the well;

    identifying, based on one or more images of the well captured at a second time substantially later than the first time, a location of a candidate colony of cells;

    determining, based on the volumetric image stack, a measure of likelihood that the candidate colony is monoclonal; and

    in response to the determined measure of likelihood, picking the candidate colony using a picking head of the apparatus to isolate at least a portion of the colony.

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