Differentiation Of Immunocytes Using Pluripotent Stem Cells
First Claim
1. A method for inducing the differentiation of hematopoietic stem cells, characterized in that the method comprisesa step of culturing pluripotent stem cells in a medium for inducing the differentiation of hematopoietic stem cells that comprises BMP4;
- 2) a step of culturing the pluripotent stem cells by treating the pluripotent stem cells with VEGF and SCF; and
3) a step of treating the pluripotent stem cells with TPO, IL-6, IL-3, and Flt3.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention relates to a method for inducing the differentiation of hematopoietic stem cells or macrophages by using pluripotent stem cells, and a composition for inducing the differentiation. Specifically, the present invention relates to a composition for inducing the differentiation of hematopoietic stem cells comprising bone morphogenetic protein 4 (BMP4) in pluripotent stem cells, or a composition for inducing the differentiation of macrophages comprising BMP4 and a macrophage-colony stimulating factor (M-CSF), and a method for inducing the differentiation by using the compositions. The method for inducing the differentiation of the present invention is advantageous in that a differentiation method thereof is simple since only a few types of cytokine are used, and compared to conventional methods for inducing the differentiation, the efficiency of differentiation is improved tens of times or more and the yield of differentiated cells is high.
-
Citations
13 Claims
-
1. A method for inducing the differentiation of hematopoietic stem cells, characterized in that the method comprisesa step of culturing pluripotent stem cells in a medium for inducing the differentiation of hematopoietic stem cells that comprises BMP4;
-
2) a step of culturing the pluripotent stem cells by treating the pluripotent stem cells with VEGF and SCF; and 3) a step of treating the pluripotent stem cells with TPO, IL-6, IL-3, and Flt3. - View Dependent Claims (2, 3, 4, 5, 6, 9)
-
-
7. A method for inducing the differentiation of macrophages, comprisinga step of culturing pluripotent stem cells in a medium for inducing the differentiation of hematopoietic stem cells that comprises BMP4;
-
2) a step of culturing the pluripotent stem cells by treating the pluripotent stem cells with VEGF and SCF; 3) a step of inducing the differentiation of hematopoietic stem cells by treating the pluripotent stem cells with TPO, IL-6, IL-3, and Flt3; and 4) a step of culturing the differentiated hematopoietic stem cells by adding the differentiated hematopoietic stem cells to a medium for inducing the differentiation of macrophages that comprises M-CSF. - View Dependent Claims (8, 10)
-
- 11. A composition for inducing the differentiation of hematopoietic stem cells or macrophages derived from pluripotent stem cells, comprising one or more selected from the group consisting of bone morphogenetic protein 4 (BMP4), vascular endothelial growth factor (VEGF), stem cell factor (SCF), platelet growth factor (thrombopoietin, TPO), interleukin-6 (IL-6), interleukin-3 (IL-3), CDDO methyl ester, FMS-like tyrosine kinase (FMS-like tyrosine kinase 3, Flt3) and macrophage-colony stimulating factor (M-CSF).
Specification