COMPOSITION CONTAINING C2CL ENDONUCLEASE FOR DIELECTRIC CALIBRATION AND METHOD FOR DIELECTRIC CALIBRATION USING SAME

• US 20200131487A1
• Filed: 03/06/2018
• Published: 04/30/2020
• Est. Priority Date: 03/06/2017
• Status: Active Application

• Current Assignee: Institute for Basic Science
• Original Assignee: Institute for Basic Science
• Inventors: KIM, Jin-Soo, KIM, Jung Eun
• Application Number:
  US16/491,208
• Publication Number:
  US 20200131487A1
• Time in Patent Office: Days
• CPC Class Codes: A01K 2217/07 Animals genetically altered by homologous recombination
• CPC Class Codes: A01K 67/0275 Genetically modified vertebrates, e.g. transgenic
• CPC Class Codes: C12N 15/11 DNA or RNA fragments; Modified forms thereof DNA or RNA not used in recombinant technology, C07H21/00; Non-coding nucleic acids having a biological activity
• CPC Class Codes: C12N 15/90 Stable introduction of foreign DNA into chromosome
• CPC Class Codes: C12N 15/907 in mammalian cells
• CPC Class Codes: C12N 2310/20 involving clustered regularly interspaced short palindromic repeats [CRISPRs]
• CPC Class Codes: C12N 2800/80 Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
• CPC Class Codes: C12N 9/22 Ribonucleases RNAses, DNAses catalytic nucleic acids C12N15/113

Abstract

Provided is a dielectric calibration technique using C2c1 endonuclease. The dielectric calibration technique is characterized by being especially applicable to eukaryotic cells, for example, to mammalian cells.

Claims

1. A genome editing composition, comprising:

a C2c1 endonuclease, a gene coding therefor, or an expression vector carrying the gene; and

a guide RNA, a DNA coding therefor, or an expression vector carrying the DNA.

2. The genome editing composition of claim 1, wherein the C2c1 endonuclease is derived from Alicyclobacillus acidoterrestris.

3. The genome editing composition of claim 2, wherein the guide RNA is represented by the following General Sequence Formula 1 or is a mutant resulting from the deletion of 1-15 nucleotides from the sequence of General Sequence Formula 1:

4. The genome editing composition of claim 3, wherein the deletion is performed on 1-15 nucleotides selected from the sequence AGCUUCUCAAA in General Sequence Formula 1.

5. The genome editing composition of claim 3, wherein the guide RNA is represented by the following General Sequence Formula 2:

6. The genome editing composition of claim 5, wherein n is 20.

7. The genome editing composition of claim 1, wherein the C2c1 endonuclease further comprises a nuclear localization signal.

8. The genome editing composition of claim 1, wherein the genome editing composition is applied to an eukaryotic cell or an eukaryotic organism.

9. A genome editing method, comprising a step of introducing the genome editing composition of claim 1 into an isolated cell or organisms exclusive of humans.

10. The genome editing method of claim 9, wherein the cell is an eukaryotic cell and the organism is an eukaryotic organism exclusive of humans.

11. An isolated genetically modified cell, having the genome editing composition of claim 1 introduced thereinto.

12. A genetically modified organism, exclusive of a human, having the genome editing composition of claim 1 introduced thereinto.