FIXED TUBE OF NUCLEIC ACID EXTRACTION COMPONENT, AND NUCLEIC ACID EXTRACTION COMPONENT

• US 20200131503A1
• Filed: 06/05/2018
• Published: 04/30/2020
• Est. Priority Date: 06/30/2017
• Status: Active Grant

• Current Assignee: CatchGene Co., Ltd.
• Original Assignee: CatchGene Co., Ltd.
• Inventors: LEE, TE-CHENG
• Granted Patent: US 11,365,406 B2
• Time in Patent Office: Days
• CPC Class Codes: B01D 2313/06 External membrane module supporting or fixing means
• CPC Class Codes: B01D 2313/205 characterised by the shape
• CPC Class Codes: B01D 2323/20 Plasticizers
• CPC Class Codes: B01D 2325/16 Membrane materials having positively charged functional groups
• CPC Class Codes: B01D 63/087 Single membrane modules
• CPC Class Codes: B01D 63/16 Rotary, reciprocated or vibrated modules
• CPC Class Codes: B01L 2200/0631 Purification arrangements, e.g. solid phase extraction [SPE]
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/043 Hinged closures
• CPC Class Codes: B01L 2300/06 Auxiliary integrated devices, integrated components
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 3/5021 Test tubes specially adapted for centrifugation purposes centrifuges B04B5/04
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: C12N 15/1017 by filtration, e.g. using filters, frits, membranes
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence

Abstract

A fixed tube of a nucleic acid extraction component and a nucleic acid extraction component. The nucleic acid extraction component includes a membrane column, which is fitted over the fixed tube. The fixed tube has a tube body, a tube opening, and a bottom. The tube body extends along a first direction. The end of the tube opening distal from the tube body has a protrusion which protrudes along a second direction, the first direction being vertical to the second direction. The bottom and the tube opening are respectively connected to two opposite sides of the tube body. The bottom has a shoulder and the shoulder of the bottom is connected to the membrane column.

Claims

1. A fixed tube of a nucleic acid extraction component, wherein the nucleic acid extraction component comprises a membrane column fitted in the fixed tube, wherein the fixed tube comprises:

a tube body extending along a first direction;

a tube opening portion, wherein one end of the tube opening portion distal from the tube body is provided with a protrusion, which protrudes along a second direction, and the first direction is perpendicular to the second direction; and

a bottom portion, wherein the bottom portion and the tube opening portion are respectively connected to two opposite sides of the tube body, the bottom portion is provided with a shoulder, and the shoulder of the bottom portion is connected to the membrane column.

2. The fixed tube of claim 1, wherein each of two opposite sides of the tube opening portion is configured with a first opening, and the first openings divide the protrusion into two sections.

3. The fixed tube of claim 2, wherein the bottom portion further comprises a stepwise structure and a fourth opening, the stepwise structure extends from the fourth opening along the first direction, the stepwise structure is connected with the membrane column, and the membrane column protrudes beyond the fixed tube through the stepwise structure.

4. The fixed tube of claim 3, wherein a diameter of the tube body is greater than a diameter of the stepwise structure.

5. The fixed tube of claim 3, wherein a diameter of the stepwise structure is gradually decreased in a direction departing from the tube body.

6. The fixed tube of claim 3, wherein the tube body is configured with a second opening, and the second opening extends along the first direction from one of the first openings to the stepwise structure.

7. The fixed tube of claim 3, wherein the fixed tube comprises a third opening, and the third opening extends along the first direction from the tube opening portion to the stepwise structure.

8. The fixed tube of claim 1, wherein the membrane column comprises a first top portion, and the shoulder of the fixed tube is fitted over an outer periphery of the first top portion of the membrane column.

9. A nucleic acid extraction component, wherein the nucleic acid extraction component comprises:

a membrane column; and

a fixed tube of claim 1.

10. The nucleic acid extraction component of claim 9, wherein the protrusion of the fixed tube contacts against an opening of a centrifugal tube.

11. The nucleic acid extraction component of claim 9, wherein each of two opposite sides of the tube opening portion is configured with a first opening, and the first openings divide the protrusion into two sections.

12. The nucleic acid extraction component of claim 11, wherein the bottom portion further comprises a stepwise structure and a fourth opening, the stepwise structure extends from the fourth opening along the first direction, the stepwise structure is connected with the membrane column, and the membrane column protrudes beyond the fixed tube through the stepwise structure.

13. The nucleic acid extraction component of claim 12, wherein a diameter of the tube body is greater than a diameter of the stepwise structure.

14. The nucleic acid extraction component of claim 12, wherein a diameter of the stepwise structure is gradually decreased in a direction departing from the tube body.

15. The nucleic acid extraction component of claim 12, wherein the tube body is configured with a second opening, and the second opening extends along the first direction from one of the first openings to the stepwise structure.

16. The nucleic acid extraction component of claim 12, wherein the fixed tube comprises a third opening, and the third opening extends along the first direction from the tube opening portion to the stepwise structure.

17. The nucleic acid extraction component of claim 9, wherein the membrane column comprises a first top portion, and the shoulder of the fixed tube is fitted over an outer periphery of the first top portion of the membrane column.