Compositions and Methods of Chromosomal Silencing
First Claim
Patent Images
1. A method for silencing genes expressed from a synthetic chromosome comprising:
- providing mammalian target cells;
transfecting the mammalian target cells with a synthetic chromosome comprising at least one stably-integrated expression cassette expressing gene products from Xic; and
expressing the gene products from Xic, wherein the synthetic chromosome is inactivated andexpression of the gene products expressed from the synthetic chromosome are silenced.
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention encompasses compositions and methods to create a whole chromosome regulatable “kill” switch to inactivate expression of one or more therapeutic genes expressed from a synthetic chromosome.
-
Citations
30 Claims
-
1. A method for silencing genes expressed from a synthetic chromosome comprising:
-
providing mammalian target cells; transfecting the mammalian target cells with a synthetic chromosome comprising at least one stably-integrated expression cassette expressing gene products from Xic; and expressing the gene products from Xic, wherein the synthetic chromosome is inactivated and expression of the gene products expressed from the synthetic chromosome are silenced. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
-
- 18. A synthetic chromosome comprising a whole chromosome kill switch.
-
30. A method for silencing genes expressed from a synthetic chromosome comprising:
-
providing mammalian target cells; transfecting the mammalian target cells with a synthetic chromosome comprising at least one stably-integrated expression cassette expressing gene products from Xic; expressing the gene products from Xic; and inactivating the synthetic chromosome where expression of the gene products expressed from the synthetic chromosome are silenced.
-
Specification