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METHOD FOR PRODUCING THEANINE

  • US 20200131546A1
  • Filed: 04/12/2018
  • Published: 04/30/2020
  • Est. Priority Date: 04/13/2017
  • Status: Active Grant
First Claim
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1. A microorganism producing acetaldehyde, alanine, glutamic acid, and ATP from a carbon source and having enhanced activity of a protein of any one of the following [1] to [3] and enhanced γ

  • -glutamylmethylamide synthetase activity compared to those of a parent strain;

    [1] a protein comprising an amino acid sequence set forth in SEQ ID NO;

    2, 4, 6, or 8;

    [2] a mutant protein comprising an amino acid sequence modified from an amino acid sequence set forth in SEQ ID NO;

    2, 4, 6, or 8 by deletion, substitution, insertion, or addition of 1 to 20 amino acids, and having activity to produce ethylamine with acetaldehyde and alanine as substrates (hereinafter, referred to as ethylamine-producing activity); and

    [3] a homologous protein comprising an amino acid sequence having 95% or more identity with an amino acid sequence set forth in SEQ ID NO;

    2, 4, 6, or 8, and having ethylamine-producing activity.

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