SYSTEM FOR PRODUCTION OF ADENOSINE TRIPHOSPHATE

• US 20200131548A1
• Filed: 12/30/2019
• Published: 04/30/2020
• Est. Priority Date: 10/17/2007
• Status: Active Grant

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Travis, Alexander
• Granted Patent: US 11,753,666 B2
• Time in Patent Office: Days
• CPC Class Codes: C12P 19/32 having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide

Abstract

The present invention relates to a system for production of ATP. This system is comprised of a support and one or more enzymes coupled to that support which are capable of collectively producing ATP from glucose or fructose metabolism. The present invention is additionally directed to a device, which includes the system, and to a method for carrying out a reaction involving the conversion of ATP to ADP using the system.

Claims

1-10. -10. (canceled)

11. A device for carrying out a reaction involving the conversion of adenosine triphosphate to adenosine diphosphate, said device comprising:

a system for production of adenosine triphosphate, said system comprising;

a support and

one or more enzymes capable of collectively producing adenosine triphosphate from glucose or fructose metabolism, wherein said one or more enzymes are coupled to said support and

components associated with said system and capable of converting adenosine triphosphate to adenosine diphosphate and carrying out the reaction.

12-22. -22. (canceled)

23. A method for carrying out a reaction involving the conversion of adenosine triphosphate to adenosine diphosphate, said method comprising:

providing a system for production of adenosine triphosphate from glucose or fructose metabolism, said system comprising;

a support and

one or more enzymes capable of collectively producing adenosine triphosphate from glucose or fructose metabolism, wherein said one or more enzymes are coupled to said support;

contacting the system with glucose or fructose under conditions effective to produce adenosine triphosphate from glucose or fructose metabolism; and

converting the adenosine triphosphate to adenosine diphosphate and carrying out the reaction.

24. The method of claim 23 further comprising:

conveying glucose or fructose to the system.

25. The method of claim 23 further comprising:

conveying pyruvate and/or lactate from the system.

26. The method of claim 23 further comprising:

conveying a substrate to the support and

removing from the support a reaction product produced from the substrate as a result of the conversion of adenosine triphosphate to adenosine diphosphate.

27. The method of claim 26, wherein the reaction product is a phosphorylated form of the substrate produced by an enzyme positioned on the support to phosphorylate the substrate.

28. The method of claim 23 further comprising:

releasing an agent from the device as a result of the adenosine triphosphate interacting with a member of an adenosine triphosphate binding cassette family of transporters.

29. The method of claim 23, wherein said one or more enzymes are hexokinase, glucose 6-phosphate isomerase, 6-phosphofructokinase, aldolase, triose-phosphate-isomerase, glyceraldehyde-3-phosphate-dehydrogenase, phosphoglycerate kinase, phosphoglycerate mutase, enolase, and pyruvate kinase.

30. The method of claim 29, wherein a plurality of the enzymes are coupled serially to the support in positions selected to serially carry out the steps of producing adenosine triphosphate from glucose or fructose metabolism.

31. The method of claim 23 further comprising:

converting pyruvate to lactate with a lactate dehydrogenase coupled to the support.

32. The method of claim 23, wherein said one or more enzymes are coupled to the support by replacing targeting/scaffolding domains of said one or more enzymes with domains capable of targeting said one or more enzymes to the support.

33. The method of claim 32, wherein the targeting/scaffolding domains of said one or more enzymes are replaced with a histidine tag and the support is provided with a linking agent which binds to the histidine tag and/or said one or more enzymes are replaced with a GST fusion tag and the support is provided with a linking agent which binds to the GST fusion tag.

34. The method of claim 33, wherein the linking agent is nickel nitrilotriacetate or glutathione.

35. The method of claim 23, wherein the support is coated with polyethylene glycol or gold.

36. The method of claim 23, wherein the system is initially primed with NAD⁺, ADP, and/or ATP.

37. The method of claim 23, wherein said reaction moves hydrogen across a membrane to produce electricity.

38. The method of claim 37 further comprising:

converting the energy in adenosine triphosphate to power an external electrical circuit.

39. The method of claim 23, wherein said reaction produces hydrogen, said method further comprising:

using the hydrogen to facilitate transport across a membrane.

40. A method for producing adenosine triphosphate, said method comprising:

providing a system for production of adenosine triphosphate from glucose or fructose metabolism, said system comprising;

a support and

one or more enzymes capable of collectively producing adenosine triphosphate from glucose or fructose metabolism, wherein said one or more enzymes are coupled to said support; and

contacting the system with glucose or fructose under conditions effective to produce adenosine triphosphate from glucose or fructose metabolism.