IVERMECTIN B1B PRODUCING STRAIN AND USE THEREOF

• US 20200131552A1
• Filed: 03/13/2018
• Published: 04/30/2020
• Est. Priority Date: 03/13/2017
• Status: Active Application

• Current Assignee: Zhejiang Hisun Pharmaceutical Company Limited (Hisun Pharmaceutical (Hangzhou) Co., Ltd.)
• Original Assignee: Zhejiang Hisun Pharmaceutical Company Limited (Hisun Pharmaceutical (Hangzhou) Co., Ltd.)
• Inventors: HUANG, Jun, LI, Na, LIN, Jiatan, LI, Meihong, ZHOU, Jun, CHANG, Zongming
• Application Number:
  US16/494,262
• Publication Number:
  US 20200131552A1
• Time in Patent Office: Days
• CPC Class Codes: A01N 43/16 with oxygen as the ring hetero atom
• CPC Class Codes: A61K 31/7048 having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin , digitoxin or digoxin
• CPC Class Codes: A61P 33/14 Ectoparasiticides, e.g. scabicides
• CPC Class Codes: C12N 1/20 Bacteria; Culture media therefor
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12P 19/445 The saccharide radical is condensed with a heterocyclic radical, e.g. everninomycin, papulacandin
• CPC Class Codes: C12P 19/623 Avermectin; Milbemycin; Ivermectin; C-076
• CPC Class Codes: C12R 2001/465 Streptomyces

Abstract

Provided are a Streptomyces avermitilis strain C63-51 with a high yield of ivermectin B1b and a method for producing ivermectin B1b by using this strain. By using the above-mentioned strain and method, efficient production of single ivermectin B1b can be achieved.

Claims

1. A Streptomyces avermitilis strain C63-51, deposited on Dec. 22, 2016 in the China General Microbiological Culture Collection Center (CGMCC) with an accession number CGMCC NO. 13370.

2. A composition comprising the Streptomyces avermitilis strain C63-51 according to claim 1.

3. A method of manufacturing ivermectin B1b or a pharmaceutical composition containing ivermectin B1b comprising using the Streptomyces avermitilis strain C63-51 according to claim 1.

4. A method of producing ivermectin B1b comprising performing fermentation in a medium containing an assimilable carbon source and/or nitrogen source using the Streptomyces avermitilis strain C63-51 according to claim 1.

5. The method according to claim 4, wherein the assimilable carbon source is one or more of sucrose, glucose, amylase, fructose, rhamnose, raffinose, xylose, arabinose, industrial molasses, lactose, galactose, maltose, trehalose, xylan, dextrin, corn starch, sorbitol, salicin, inositol, mannitol, glycerol, glycine or inulin.

6. The method according to claim 4, wherein the assimilable nitrogen source is one or more of beef extractum, yeast extractum, yeast extract, yeast powder, peptone, tryptone, gluten powder, cottonseed meal, peanut meal, soybean meal, dried powder of corn steep liquor, bran, urea, ammonium salt or nitrate.

7. The method according to claim 4;

wherein the medium further comprises an inorganic salt, and the inorganic salt is one or more of MnSO₄, Na₂MoO₄, CoCl₂, CaCO₃, ZnSO₄, FeSO₄, (NH₄)₂SO₄, FeCl₃, KNO₃, KH₂PO₄, K₂HPO₄, MgCl₂, MgSO₄, NaCl, CuSO₄, NiSO₄ or KCl.

8. The method according to claim 4, wherein the medium contains corn starch 120-160 g/L, amylase 0.2 g/L, soybean meal 10-40 g/L, peanut meal 0-10 g/L, yeast powder 5-20 g/L, (NH₄)₂SO₄ 0-4 g/L, MnSO₄ 0.024 g/L, Na₂MoO₄ 0.024 g/L, CoCl₂ 0.01-0.04 g/L and CaCO₃ 3-7 g/L.

9. The method according to claim 4, wherein the medium contains corn starch 140 g/L, amylase 0.2 g/L, soybean meal 20 g/L, peanut meal 5 g/L, yeast powder 10 g/L, (NH₄)₂SO₄ 2 MnSO₄ 0.024 g/L, Na₂MoO₄ 0.024 g/L, CoCl₂ 0.02 g/L and CaCO₃ 7 g/L.

10. The method according to claim 4, wherein the temperature of the fermentation is 20 to 40°

C.

11. The method according to claim 4, wherein the pH of the medium is 6.0-8.0.

12. The method according to claim 4, wherein the duration of the fermentation is 288-312 hours, and/or with a ventilation of 0.6-1.1 vvm.

13. The method according to claim 4, wherein the temperature of the fermentation is 25 to 30°

C., preferably 28°

C.;

the pH of the medium is 6.5-7.5, preferably 7.0-7.2.

14. An pesticidal composition comprising ivermectin B1b produced by the method according to claim 4.